Multiplex Fourier-transform infrared microscopy (μFT-IR) helped to monitor trans-[Ru(NO) (NH3)4 (isn)]3+(I), uptake by A549 lung carcinoma cell, as well as the generation of its product, nitric oxide (NO), inside the cell. Chronoamperometry with NO-sensor and μFT-IR showed that exogenous NADH and the A549 cell induced the NO release redox mechanism. Chemical imaging confirmed that (I) was taken up by the cell, and that its localization coincided with its consumption in the cellular environment within 15 min of exposure. The Ru-NO absorption band in the IR spectrum shifted from 1932 cm-1, when NO was coordinated to Ru as {RuII-NO+}3+, to 1876 cm-1, due the formation of reduced species {RuII-NO0}2+, a precursor of NO release. Futhermore, the μFT-IR spectral profile demonstrated that, as a result of the NO action on the target, NO interacted with nucleic acids, which provided a biochemical response that is detectable in living cells.

中文翻译：

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使用多重傅里叶变换红外显微镜对单个A549癌细胞中用作NO供体的亚硝酰基钌络合物进行实时氧化还原监测。

多重傅里叶变换红外显微镜（μFT-IR）有助于监测反式[Ru（NO）（NH3）4（isn）] 3+（I），A549肺癌细胞的摄取及其产物的产生电池内部的一氧化氮（NO）。用NO传感器和μFT-IR进行的计时电流分析表明，外源NADH和A549细胞诱导NO释放氧化还原机制。化学成像证实（I）被细胞吸收，并且其定位与其在暴露后15分钟内在细胞环境中的消耗相吻合。由于还原物种{RuII-NO0} 2的形成，IR光谱中的Ru-NO吸收带从1932 cm-1（当NO与Ru配成{RuII-NO +} 3+时）移动到1876 cm-1。 +，NO释放的前兆。此外，μFT-IR光谱图表明，由于NO对目标的作用，