Growth cones (GCs) are structures associated with growing neurons. GC membrane expansion, which necessitates protein-lipid interactions, is critical to axonal elongation in development and in adult neuritogenesis. We present a multi-omic analysis that integrates proteomics and lipidomics data for the identification of GC pathways, cell phenotypes, and lipid-protein interactions, with an analytic platform to facilitate the visualization of these data. We combine lipidomic data from GC and adult axonal regeneration following optic nerve crush. Our results reveal significant molecular variability in GCs across developmental ages that aligns with the upregulation and downregulation of lipid metabolic processes and correlates with distinct changes in the lipid composition of GC plasmalemma. We find that these processes also define the transition into a growth-permissive state in the adult central nervous system. The insight derived from these analyses will aid in promoting adult regeneration and functional innervation in devastating neurodegenerative diseases.

生长锥 (GC) 是与生长神经元相关的结构。GC 膜膨胀需要蛋白质-脂质相互作用，对于发育和成人神经发生过程中的轴突伸长至关重要。我们提出了一个多组学分析，它整合了蛋白质组学和脂质组学数据，用于识别 GC 途径、细胞表型和脂质 - 蛋白质相互作用，并通过一个分析平台来促进这些数据的可视化。我们将 GC 的脂质组学数据与视神经挤压伤后的成人轴突再生相结合。我们的结果揭示了 GCs 在整个发育年龄中的显着分子变异性，这与脂质代谢过程的上调和下调一致，并与 GC 质膜脂质组成的明显变化相关。我们发现这些过程也定义了成人中枢神经系统向生长允许状态的过渡。从这些分析中获得的见解将有助于促进破坏性神经退行性疾病中的成人再生和功能神经支配。