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Specific Ion Channels Control Sensory Gain, Sensitivity, and Kinetics in a Tonic Thermonociceptor.
Cell Reports ( IF 7.5 ) Pub Date : 2020-01-14 , DOI: 10.1016/j.celrep.2019.12.029
Gabriella Saro 1 , Andrei-Stefan Lia 1 , Saurabh Thapliyal 1 , Filipe Marques 1 , Karl Emanuel Busch 2 , Dominique A Glauser 1
Affiliation  

Pain sensation and aversive behaviors entail the activation of nociceptor neurons, whose function is largely conserved across animals. The functional heterogeneity of nociceptors and ethical concerns are challenges for their study in mammalian models. Here, we investigate the function of a single type of genetically identified C. elegans thermonociceptor named FLP. Using calcium imaging in vivo, we demonstrate that FLP encodes thermal information in a tonic and graded manner over a wide thermal range spanning from noxious cold to noxious heat (8°C-36°C). This tonic-signaling mode allows FLP to trigger sustained behavioral changes necessary for escape behavior. Furthermore, we identify specific transient receptor potential, voltage-gated calcium, and sodium "leak" channels controlling sensory gain, thermal sensitivity, and signal kinetics, respectively, and show that the ryanodine receptor is required for long-lasting activation. Our work elucidates the task distribution among specific ion channels to achieve remarkable sensory properties in a tonic thermonociceptor in vivo.

中文翻译:

特定的离子通道控制补品热伤害感受器中的感官增益,灵敏度和动力学。

疼痛感和厌恶行为需要伤害感受神经元的激活,伤害感受神经元的功能在整个动物中都非常保守。伤害感受器的功能异质性和伦理问题是他们在哺乳动物模型中进行研究的挑战。在这里,我们调查了一种名为FLP的遗传鉴定的秀丽隐杆线虫热伤害感受器的单一类型的功能。使用体内钙成像,我们证明FLP在从有毒的寒冷到有毒的热量(8°C-36°C)的宽广热范围内以补品和分级的方式编码热信息。这种强音信号模式允许FLP触发逃生行为所需的持续行为变化。此外,我们还确定了特定的瞬时受体电位,电压门控钙和钠“泄漏”通道,这些通道控制着感官增益,热敏感性和信号动力学,分别表明,对于长期激活而言,赖氨酸受体是必需的。我们的工作阐明了特定离子通道之间的任务分配,以在体内滋补热伤害感受器中实现卓越的感官特性。
更新日期:2020-01-15
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