Translation of genomic alterations to protein changes in chronic obstructive pulmonary disease (COPD) is largely unexplored. Using integrated proteomic and RNA sequencing analysis of COPD and control lung tissues, we identified a protein signature in COPD characterised by extracellular matrix changes and a potential regulatory role for SUMO2. Furthermore, we identified 61 differentially expressed novel, non-reference, peptides in COPD compared with control lungs. This included two peptides encoding for a new splice variant of SORBS1, of which the transcript usage was higher in COPD compared with control lungs. These explorative findings and integrative proteogenomic approach open new avenues to further unravel the pathology of COPD.

中文翻译：

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识别COPD的蛋白质特征和SORBS1的新剪接变体的综合蛋白质组学方法

在慢性阻塞性肺疾病 (COPD) 中，基因组改变对蛋白质变化的转化在很大程度上尚未得到探索。使用 COPD 和对照肺组织的综合蛋白质组学和 RNA 测序分析，我们确定了 COPD 中的蛋白质特征，其特征在于细胞外基质变化和 SUMO2 的潜在调节作用。此外，与对照肺相比，我们在 COPD 中鉴定了 61 种差异表达的新型非参考肽。这包括编码 SORBS1 新剪接变体的两种肽，与对照肺相比，COPD 中的转录物使用率更高。这些探索性发现和综合蛋白基因组学方法为进一步阐明 COPD 的病理学开辟了新的途径。