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Environmental DNA reveals seasonal shifts and potential interactions in a marine community.
Nature Communications ( IF 14.7 ) Pub Date : 2020-01-14 , DOI: 10.1038/s41467-019-14105-1
Anni Djurhuus 1 , Collin J Closek 2, 3 , Ryan P Kelly 4 , Kathleen J Pitz 5 , Reiko P Michisaki 5 , Hilary A Starks 2, 3 , Kristine R Walz 5 , Elizabeth A Andruszkiewicz 3 , Emily Olesin 6 , Katherine Hubbard 6 , Enrique Montes 1 , Daniel Otis 1 , Frank E Muller-Karger 1 , Francisco P Chavez 5 , Alexandria B Boehm 3 , Mya Breitbart 1
Affiliation  

Environmental DNA (eDNA) analysis allows the simultaneous examination of organisms across multiple trophic levels and domains of life, providing critical information about the complex biotic interactions related to ecosystem change. Here we used multilocus amplicon sequencing of eDNA to survey biodiversity from an eighteen-month (2015-2016) time-series of seawater samples from Monterey Bay, California. The resulting dataset encompasses 663 taxonomic groups (at Family or higher taxonomic rank) ranging from microorganisms to mammals. We inferred changes in the composition of communities, revealing putative interactions among taxa and identifying correlations between these communities and environmental properties over time. Community network analysis provided evidence of expected predator-prey relationships, trophic linkages, and seasonal shifts across all domains of life. We conclude that eDNA-based analyses can provide detailed information about marine ecosystem dynamics and identify sensitive biological indicators that can suggest ecosystem changes and inform conservation strategies.

中文翻译:


环境 DNA 揭示了海洋群落的季节变化和潜在的相互作用。



环境 DNA (eDNA) 分析可以同时检查多个营养级别和生命领域的生物体,提供有关与生态系统变化相关的复杂生物相互作用的关键信息。在这里,我们使用 eDNA 的多位点扩增子测序来调查加利福尼亚州蒙特利湾 18 个月(2015-2016)时间序列的海水样本的生物多样性。生成的数据集涵盖从微生物到哺乳动物的 663 个分类群(科或更高分类等级)。我们推断了群落组成的变化,揭示了类群之间假定的相互作用,并确定了这些群落与环境特性之间随时间的相关性。群落网络分析提供了预期的捕食者-猎物关系、营养联系以及生命各个领域的季节变化的证据。我们的结论是,基于 eDNA 的分析可以提供有关海洋生态系统动态的详细信息,并识别敏感的生物指标,从而建议生态系统变化并为保护策略提供信息。
更新日期:2020-01-14
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