Subcellular asymmetry directed by the planar cell polarity (PCP) signaling pathway orients numerous morphogenetic events in both invertebrates and vertebrates. Here, we describe a morphogenetic movement in which the intertwined socket and shaft cells of the Drosophila anterior wing margin mechanosensory bristles undergo PCP-directed apical rotation, inducing twisting that results in a helical structure of defined chirality. We show that the Frizzled/Vang PCP signaling module coordinates polarity among and between bristles and surrounding cells to direct this rotation. Furthermore, we show that dynamic interplay between two isoforms of the Prickle protein determines right- or left-handed bristle morphogenesis. We provide evidence that, Frizzled/Vang signaling couples to the Fat/Dachsous PCP directional signal in opposite directions depending on whether Pk^pk or Pk^sple predominates. Dynamic interplay between Pk isoforms is likely to be an important determinant of PCP outcomes in diverse contexts. Similar mechanisms may orient other lateralizing morphogenetic processes.

中文翻译：

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刺突亚型决定螺旋形态发生的惯用性

由平面细胞极性（PCP）信号传导途径指导的亚细胞不对称性在无脊椎动物和脊椎动物中定向了许多形态发生事件。在这里，我们描述了果蝇的交织的窝和轴细胞的形态发生运动前翼边缘机械感觉性刷毛受到PCP引导的根尖旋转，引起扭曲，从而形成确定的手性的螺旋结构。我们显示，Frizzled / Vang PCP信号模块协调刷毛与周围细胞之间以及极性之间的极性，以指导此旋转。此外，我们显示了Prickle蛋白的两个同工型之间的动态相互作用决定了右手或左手鬃毛的形态发生。我们提供的证据表明，根据Pk ^pk还是Pk^脾脏，卷曲/旺信号会以相反的方向耦合到胖/胖PCP方向信号占主导地位。在不同情况下，Pk亚型之间的动态相互作用很可能是PCP结果的重要决定因素。相似的机制可能会定向其他横向化形态发生过程。