Miniaturized fluorescence microscopes (miniscopes) have been instrumental to monitor neural signals during unrestrained behavior and their open-source versions have made them affordable. Often, the footprint and weight of open-source miniscopes is sacrificed for added functionality. Here, we present NINscope: a light-weight miniscope with a small footprint that integrates a high-sensitivity image sensor, an inertial measurement unit and an LED driver for an external optogenetic probe. We use it to perform the first concurrent cellular resolution recordings from cerebellum and cerebral cortex in unrestrained mice, demonstrate its optogenetic stimulation capabilities to examine cerebello-cerebral or cortico-striatal connectivity, and replicate findings of action encoding in dorsal striatum. In combination with cross-platform control software, our miniscope is a versatile addition to the expanding toolbox of open-source miniscopes that will increase access to multi-region circuit investigations during unrestrained behavior.

中文翻译：

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NINscope，一种用于多区域电路研究的多功能微型镜

微型荧光显微镜（微型显微镜）在无节制的行为过程中一直用于监视神经信号，并且它们的开源版本使其价格可承受。通常，为增加功能而牺牲开源微型显微镜的占地面积和重量。在这里，我们介绍了NINscope：一种占地面积小，重量轻的微型显微镜，集成了高灵敏度图像传感器，惯性测量单元和用于外部光遗传学探针的LED驱动器。我们使用它来执行不受约束的小鼠小脑和大脑皮层的第一个同时发生的细胞分辨率记录，展示其光遗传刺激能力以检查小脑-大脑或皮层-纹状体的连通性，并复制在背侧纹状体中编码作用的发现。结合跨平台控制软件，