The microalgae Aurantiochytrium sp. is a strong alternative source of ω-3 fatty acids, including docosahexaenoic acid (DHA). This work encompasses the optimization of SFE conditions to maximize the total extraction yield $\left({\eta }_{\text{T}\text{o}\text{t}\text{a}\text{l}}\right)$, DHA content ($C_{\text{D}\text{H}\text{A}}$), total phenolics content ($TPC$), and antioxidant capacity ($AOC$) of the extracts produced from Aurantiochytrium sp. biomass. A full factorial experimental plan was performed, comprising three factors (pressure, temperature, and flow rate) and two levels (200−300 bar, 40−80 °C, and 6–12 $\text{g}{\text{m}\text{i}\text{n}}^{-1}$, respectively). The maximum and minimum experimental results were ${\eta }_{\text{T}\text{o}\text{t}\text{a}\text{l}}=$ 2.1 and 13.4 wt.%, $C_{\text{D}\text{H}\text{A}}=$ 27.3 and 39.3 wt.%, $TPC$ = 1.19 and 2.24 ${\text{m}\text{g}}_{\text{G}\text{A}\text{E}}{\text{g}}_{\text{e}\text{x}\text{t}\text{r}\text{a}\text{c}\text{t}}^{-1}$, and $AOC$ = 0.3 and 1.4 ${\text{m}\text{g}}_{\text{T}\text{E}\text{A}\text{C}}{\text{g}}_{\text{e}\text{x}\text{t}\text{r}\text{a}\text{c}\text{t}}^{-1}$. Under the studied experimental conditions, increasing pressure up to 300 bar is the optimum to rise both ${\eta }_{\text{T}\text{o}\text{t}\text{a}\text{l}}$ and $C_{\text{D}\text{H}\text{A}}$. Temperature increase from 40 to 80 °C leads to opposing effects: it favors the concentration of phenolics in the supercritical extracts at the expenses of decreasing DHA content and total yield. Surface models were adjusted to ${\eta }_{\text{T}\text{o}\text{t}\text{a}\text{l}}$, $C_{\text{D}\text{H}\text{A}}$ and $TPC$ data, and the goodness of the fits ranged from coefficients of determination of 0.752-0.711 ($TPC$) to 0.997-0.994 ($C_{\text{D}\text{H}\text{A}}$). Under optimized conditions, supercritical extracts exhibited a DHA content more than 3.5-fold richer than fish oil, and 7.9-fold richer than the best alternative microalgae species (Pavlova lutheri) found in the literature.

微藻Aurantiochytrium sp。是ω-3脂肪酸（包括二十二碳六烯酸（DHA））的强大替代来源。这项工作包括优化SFE条件，以最大程度地提高总提取率$\left({\eta }_{\text{Ť}\text{Ø}\text{Ť}\text{一种}\text{升}}\right)$，DHA含量（$C_{\text{d}\text{H}\text{一种}}$），总酚含量（$ŤPC$），抗氧化能力（$\mathrm{一种}ØC$）从Aurantiochytrium sp。产生的提取物中。生物质。执行了完整的析因实验计划，包括三个因素（压力，温度和流速）和两个水平（200-300 bar，40-80°C和6-12）$\text{G}{\text{米}\text{一世}\text{ñ}}^{--\mathrm{1个}}$， 分别）。最大和最小实验结果为${\eta }_{\text{Ť}\text{Ø}\text{Ť}\text{一种}\text{升}}=$ 2.1和13.4 wt。％， $C_{\text{d}\text{H}\text{一种}}=$ 27.3和39.3 wt。％， $ŤPC$ = 1.19和2.24 ${\text{米}\text{G}}_{\text{G}\text{一种}\text{Ë}}{\text{G}}_{\text{Ë}\text{X}\text{Ť}\text{[R}\text{一种}\text{C}\text{Ť}}^{--\mathrm{1个}}$和 $\mathrm{一种}ØC$ = 0.3和1.4 ${\text{米}\text{G}}_{\text{Ť}\text{Ë}\text{一种}\text{C}}{\text{G}}_{\text{Ë}\text{X}\text{Ť}\text{[R}\text{一种}\text{C}\text{Ť}}^{--\mathrm{1个}}$。在研究的实验条件下，增加压力至300 bar是同时升高两者的最佳选择${\eta }_{\text{Ť}\text{Ø}\text{Ť}\text{一种}\text{升}}$ 和 $C_{\text{d}\text{H}\text{一种}}$。温度从40°C升高到80°C会产生相反的效果：它有利于超临界萃取物中的酚类浓度，但会降低DHA含量和总收率。曲面模型已调整为${\eta }_{\text{Ť}\text{Ø}\text{Ť}\text{一种}\text{升}}$， $C_{\text{d}\text{H}\text{一种}}$ 和 $ŤPC$ 数据，拟合优度介于0.752-0.711（$ŤPC$）到0.997-0.994（$C_{\text{d}\text{H}\text{一种}}$）。在最佳条件下，超临界提取物的DHA含量比鱼油高3.5倍，比文献中发现的最佳替代微藻物种（Pavlova lutheri）高7.9倍。