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Multi-residue Ultra Performance Liquid Chromatography-High resolution mass spectrometric method for the analysis of 21 cyanotoxins in surface water for human consumption.
Talanta ( IF 5.6 ) Pub Date : 2020-01-14 , DOI: 10.1016/j.talanta.2020.120738 Giorgia Di Pofi 1 , Gabriele Favero 2 , Federica Nigro Di Gregorio 3 , Emanuele Ferretti 3 , Emanuela Viaggiu 4 , Luca Lucentini 3
Talanta ( IF 5.6 ) Pub Date : 2020-01-14 , DOI: 10.1016/j.talanta.2020.120738 Giorgia Di Pofi 1 , Gabriele Favero 2 , Federica Nigro Di Gregorio 3 , Emanuele Ferretti 3 , Emanuela Viaggiu 4 , Luca Lucentini 3
Affiliation
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The presence of cyanobacteria and their toxins in water used as drinking water or for recreational purposes may represent a risk for human health. This work describes the development of an advanced analytical method for simultaneous determination of 21 cyanotoxins (including Microcystins, Cyanopeptolins, Anabaenopeptins and Microginins) in drinking water based on Ultra Performance Liquid Chromatography coupled with a Q-TOF mass spectrometer. Water samples, spiked with Nodularin as internal standard at 1 μg/L, were extracted using Carbograph 4 SPE cartridge and 10 μL of the extracted sample were injected into the UPLC-HRMS/MS system. Analytes separation was obtained using a UPLC C18 column, acetonitrile and water as mobile phases, both containing 10 mM formic acid, and operating in positive ionization mode and sensitivity mode. The method has been proven to be robust, precise and accurate with recovery percentages above 85% and with relative standard deviations ≤16% and LODs between 0.002 and 0.047 μg/L, fitting for the intended purposes at the concentrations of interest. This method was applied during a monitoring activity in an Italian volcanic lake in Viterbo (Lazio Region, Italy), due to a severe algal proliferation in January 2018-March 2019 period and for the assessment of cyanobacteria proliferation risk and of cyanotoxin production in drinking water chain.
中文翻译：
多残留超高效液相色谱-高分辨率质谱法分析供人类食用的地表水中的21种氰毒素。
用作饮用水或用于娱乐目的的水中存在蓝细菌及其毒素可能会对人体健康构成威胁。这项工作描述了一种基于超高效液相色谱和Q-TOF质谱仪同时测定饮用水中21种氰毒素（包括微囊藻毒素，氰基色氨酸，Anabaenopeptins和Microginins）的先进分析方法的开发。使用Carbograph 4 SPE小柱提取水样，以Nodularin作为内标加标1μg/ L，然后将提取的10μL样品注入UPLC-HRMS / MS系统。使用UPLC C18色谱柱，乙腈和水作为流动相进行分析物分离，均包含10 mM甲酸，并以正电离模式和灵敏度模式运行。该方法已被证明是可靠，准确和准确的方法，回收率高于85％，相对标准偏差≤16％，LOD在0.002至0.047μg/ L之间，适合目标浓度的目的。由于2018年1月至2019年3月期间严重的藻类繁殖，该方法在维泰博（意大利拉齐奥地区）的意大利火山湖的监测活动中使用，用于评估蓝藻繁殖风险和饮用水中氰毒素的产生链。
更新日期：2020-01-14
中文翻译：
多残留超高效液相色谱-高分辨率质谱法分析供人类食用的地表水中的21种氰毒素。
用作饮用水或用于娱乐目的的水中存在蓝细菌及其毒素可能会对人体健康构成威胁。这项工作描述了一种基于超高效液相色谱和Q-TOF质谱仪同时测定饮用水中21种氰毒素（包括微囊藻毒素，氰基色氨酸，Anabaenopeptins和Microginins）的先进分析方法的开发。使用Carbograph 4 SPE小柱提取水样，以Nodularin作为内标加标1μg/ L，然后将提取的10μL样品注入UPLC-HRMS / MS系统。使用UPLC C18色谱柱，乙腈和水作为流动相进行分析物分离，均包含10 mM甲酸，并以正电离模式和灵敏度模式运行。该方法已被证明是可靠，准确和准确的方法，回收率高于85％，相对标准偏差≤16％，LOD在0.002至0.047μg/ L之间，适合目标浓度的目的。由于2018年1月至2019年3月期间严重的藻类繁殖，该方法在维泰博（意大利拉齐奥地区）的意大利火山湖的监测活动中使用，用于评估蓝藻繁殖风险和饮用水中氰毒素的产生链。
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