Reverse micellar extraction (RME) has emerged as a versatile and efficient tool for downstream processing (DSP) of various biomolecules, including structural proteins and enzymes, due to the substantial advantages over conventional DSP methods. However, the RME system is a complex dependency of several parameters that influences the overall selectivity and performance of the RME system, hence this justifies the need for optimization to obtain higher possible extraction results. For the last two decades, many experimental design strategies for screening and optimization of RME have been described in literature. The objective of this article is to review the use of different experimental designs and response surface methodologies that are currently used to screen and optimize the RME system for various types of biomolecules. Overall, this review provides the rationale for the selection of appropriate screening or optimization techniques for the parameters associated with both forward and backward extraction during the RME of biomolecules.

中文翻译：

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实验设计和响应面方法在反胶束萃取筛选和优化中的应用。

逆胶束提取（RME）已成为一种通用且有效的工具，可用于各种生物分子（包括结构蛋白和酶）的下游加工（DSP），这是其与常规DSP方法相比的显着优势。但是，RME系统是几个参数的复杂依赖性，这会影响RME系统的整体选择性和性能，因此这证明需要进行优化以获得更高可能的提取结果是合理的。在过去的二十年中，文献中描述了许多用于筛选和优化RME的实验设计策略。本文的目的是回顾当前用于筛选和优化各种类型生物分子的RME系统的不同实验设计和响应面方法的使用。总体，