In view of large-scale production, usage and toxic nature, the synthetic dyes are a very significant category of pollutants. This work reports the preparation and application of reduced graphene oxide/cobalt oxide (rGO/Co₃O₄) nanocomposite as selective and regenerable magnetite nanosorbent for the preconcentration and determination of brilliant green (BG) as a synthetic dye by UV–Vis spectrophotometry. The properties of the rGO/Co₃O₄ nanocomposite were characterized by UV–Vis spectrophotometry, Fourier-transform infrared spectroscopy, X-ray diffraction, field emission scanning electron microscopy, vibrating sample magnetometer and inductively coupled plasma optical emission spectrometry. Magnetic dispersive microsolid phase extraction (MD-μSPE) as a green, simple and sensitive method has been proposed for the separation and determination of trace quantities of BG. The experimental parameters affecting the MD-μSPE efficiency for the target analytes such as sample pH, brilliant green concentration, adsorbent dose and sonication time were investigated and optimized by central composite design coupled with desirability function approach. Under the optimized conditions, the detection limit, relative standard deviation and enrichment factor were 2.6 ng mL⁻¹, 3.34% (n = 5) and 24.5, respectively. Finally, the applicability of the proposed method was successfully evaluated by the determination of trace amounts of BG in fish breeding pool and tap water samples.

考虑到大规模生产，使用和毒性性质，合成染料是非常重要的污染物类别。这项工作报告了还原型氧化石墨烯/氧化钴（rGO / Co ₃ O ₄）纳米复合物的制备和应用，该纳米复合物作为选择性和可再生的磁铁矿纳米吸附剂，用于紫外-可见分光光度法的富集和测定作为合成染料的亮绿色（BG）。rGO / Co ₃ O _4的性质纳米复合材料的特征在于紫外可见分光光度法，傅立叶变换红外光谱，X射线衍射，场发射扫描电子显微镜，振动样品磁强计和电感耦合等离子体发射光谱法。磁性分散微固相萃取（MD-μSPE）是一种绿色，简单而灵敏的方法，已被提出用于分离和测定痕量的BG。通过中央复合设计结合期望函数方法，研究并优化了影响目标分析物的MD-μSPE效率的实验参数，例如样品pH，亮绿色浓度，吸附剂剂量和超声处理时间。在最佳条件下，检出限，相对标准偏差和富集因子均为2.6 ng mL ^-1，3.34％（n  = 5）和24.5。最后，通过测定鱼类繁殖池和自来水样品中痕量的BG，成功评估了该方法的适用性。