Bacillus amyloliquefaciens has been widely used as a probiotic in the field of biological control,and its antibacterial compounds plays an important role in the prevention and control of plant, livestock and poultry diseases. It has the advantages of green, safe and efficiency. This study aims to separate and purify active ingredient from Bacillus amyloliquefaciens GN59 and study its antibacterial activity. A novel compound was isolated from GN59 by column chromatography on silica gel and HPLC purification. The chemical structure was identified as α-D-glucopyranosyl-(1 → 1')-3'-amino-3'-deoxy-β-D-glucopyranoside (a,β-3-trehalosamine) on the basis of spectroscopic analysis. This is the first report about a,β-3-trehalosamine isolated from biological resources on an antibiotic activity against pathogenic bacterium. The 3'-neotrehalosamine displayed antibacterial activity across a broad spectrum of microorganisms, including different gram-positive and gram-negative bacteria, with minimal inhibitory concentration (MIC) values ranging from 0.5 to 0.7 mg/mL. The results indicated that the 3'-neotrehalosamine from GN59 might be a potential candidate for bactericide.

中文翻译：

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从解淀粉芽孢杆菌分离的新型海藻糖胺及其抗菌活性。

淀粉芽孢杆菌已被广泛用作生物防治领域中的益生菌，其抗菌化合物在植物，畜禽疾病的预防和控制中起着重要的作用。具有绿色，安全，高效的优点。本研究旨在从解淀粉芽孢杆菌GN59中分离和纯化活性成分，并研究其抗菌活性。通过硅胶柱色谱法和HPLC纯化从GN59中分离出一种新化合物。在光谱分析的基础上，化学结构鉴定为α-D-吡喃葡萄糖基-（1→1'）-3'-氨基-3'-脱氧-β-D-吡喃葡萄糖苷（a，β-3-海藻糖胺）。这是关于从生物资源中分离出α，β-3-海藻胺对致病菌的抗生素活性的首次报道。3' -neorehalosamines在广泛的微生物（包括不同的革兰氏阳性和革兰氏阴性细菌）中显示出抗菌活性，其最小抑菌浓度（MIC）值在0.5至0.7 mg / mL范围内。结果表明，来自GN59的3'-新卤代卤胺可能是杀菌剂的潜在候选者。