Increasing evidences suggest that intestinal microbiota balance closely correlated with host's health status could affected by external environment. Integrated crayfish-rice cultivation model is a highly efficient artificial ecosystem widely practiced in subtropical China. Less information is available to estimate the influence response to the micro-ecology of crayfish intestine and so as to influence the biological processes. Thus, 16S rRNA high-throughput sequencing approach was employed to investigate the composition diversity and functions of bacterial community in the intestines of Procambarus clarkii farmed within this model. Results exhibited the highly diversity of microflora with dominant phyla Actinobacteria, Proteobacteria, Tenericutes, Firmicutes and Bacteroidetes. The genera of Candidatus Bacilloplasma and Ornithinibacter were presented as predominant population much exceeds in richness comparing to that of other genus. Despite the highly diversity in the bacterial community, the predicted functions indicated relative consistent in biological processing pathway. Collectively, significant richness of genes was observed involved in amino acid and carbohydrate metabolism and membrane transport processing. This study would contribute to the understanding of the impact of growth conditions on host-microbiota relation especially in aquatic animals.

中文翻译：

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集成小龙虾-大米养殖模型中的红色沼泽小龙虾Procambarus clarkii的肠道菌群。

越来越多的证据表明，肠道微生物群的平衡与寄主的健康状况密切相关，可能会受到外部环境的影响。小龙虾-稻米综合养殖模式是在亚热带地区广泛应用的高效人工生态系统。较少的信息可用来估计对小龙虾肠微生态的影响响应，从而影响其生物过程。因此，采用16S rRNA高通量测序方法研究了在该模型中养殖的克氏原螯虾肠道中细菌群落的组成多样性和功能。结果显示，菌群具有高度的多样性，其中优势的是放线菌，变形杆菌，Tenericutes，Firmicutes和Bacteroidetes。与其他属相比，细菌的念珠菌属和鸟氨酸杆菌属的优势种群比其丰富度大得多。尽管在细菌群落中高度多样性，但预测的功能表明在生物加工途径中相对一致。集体地，观察到显着丰富的基因参与氨基酸和碳水化合物的代谢以及膜转运过程。这项研究将有助于了解生长条件对宿主-微生物群关系的影响，尤其是在水生动物中。观察到显着丰富的基因参与了氨基酸和碳水化合物的代谢以及膜转运过程。这项研究将有助于了解生长条件对宿主-微生物群关系的影响，尤其是在水生动物中。观察到显着丰富的基因参与了氨基酸和碳水化合物的代谢以及膜转运过程。这项研究将有助于了解生长条件对宿主-微生物群关系的影响，尤其是在水生动物中。