The extraordinary nature of the bacterial cellulose (BC) biopolymer gives it potential for diverse applications; however, the low BC yield of many indigenous cellulose-producing bacteria is a persistent problem in its synthesis. In this study, the BC yield of Komactobacter intermedius (BCRC 910677) was optimized by modifying culture media. The optimal culture period, type of carbon, and nitrogen sources were evaluated using the one-factor-at-a-time approach prior to the optimization study. The optimization was done by using the response surface methodology (RSM). In RSM optimization study, a Box–Behnken design with three parameters is applied; the three parameters include fructose concentrations (X₁), peptone concentrations (X₂), and pH values (X₃). Our optimal culture media combined 41 g/L of fructose, 38 g/L peptone, and a pH of 5.2. The predicted BC yield from the RSM model is 4.012 g/L, while BC yield of 3.906 g/L is obtained from the experiment using the optimized medium; that is only 2.64% difference. An increase in BC production of 3.82-fold (compared to the culture in HS medium) was obtained after 6-days culture. The K. intermedius investigated in this study show great potential for commercial BC productions and as feedstock. The RSM can be a promising approach to enhance BC yield since the parameters were well correlated.

中文翻译：

---

使用统计模型提高中间型嗜酸杆菌的细菌纤维素产量

细菌纤维素（BC）生物聚合物的非凡性质使其具有多种应用的潜力。然而，许多本地产生纤维素的细菌的低BC产量是其合成中的持续问题。在这项研究中，通过改良培养基优化了中间嗜酸杆菌（BCMC 910677）的BC产量。在优化研究之前，使用一次一因素法评估了最佳培养期，碳和氮源类型。通过使用响应面方法（RSM）进行了优化。在RSM优化研究中，采用了具有三个参数的Box-Behnken设计。这三个参数包括果糖浓度（X ₁），蛋白one浓度（X ₂）和pH值（X ₃）。我们的最佳培养基结合了41 g / L的果糖，38 g / L的蛋白ept和pH值为5.2。通过RSM模型预测的BC产量为4.012 g / L，而使用优化的培养基从实验中获得的BC产量为3.906 g / L。差异只有2.64％。培养6天后，BC产量增加了3.82倍（与HS培养基中的培养相比）。该K.中间型在这项研究调查显示商业BC制作和原料的巨大潜力。由于参数之间具有很好的相关性，因此RSM可能是提高BC产量的有前途的方法。