当前位置: X-MOL 学术Mater. Lett. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Rapid and Size-Controlled Biosynthesis of Cytocompatible Selenium Nanoparticles by Azadirachta indica Leaves Extract for Antibacterial Activity
Materials Letters ( IF 3 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.matlet.2020.127353
Nayeem A. Mulla , Sachin V. Otari , Raghvendra A. Bohara , Hemraj M. Yadav , Shivaji H. Pawar

Abstract Biosynthesized selenium nanoparticles (SeNPs) have achieved considerable importance in biomedical applications owing to their biocompatibility and low toxicity. In this study, rapid and size-controlled biosynthesis of SeNPs by aqueous leaves extract of Azadirachta indica is reported. SeNPs were synthesized by reduction of Se+ ions of sodium selenite salt to elemental selenium. Biosynthesized SeNPs were crystalline in nature and spherical in shape with a smooth surface. The size of these SeNPs was controlled by adjusting the time of reduction reaction. Accordingly, SeNPs of size range 142 to 168 nm and 221 to 328 nm were synthesized after 5 and 10 min of reaction respectively with 10 mM initial concentration of sodium selenite. Fourier Transform- Infra Red (FT-IR) analysis revealed the presence of plant extract molecules on the surface of SeNPs acting as reducing agent and capping molecules. These biosynthesized SeNPs were found to be cytocompatible in nature when tested in-vitro against the L929 cell line by MTT assay. In addition, these biosynthesized SeNPs showed promising antibacterial activity against selected Gram-positive and Gram-negative bacterial strains.

中文翻译:

印楝叶提取物对细胞相容性硒纳米颗粒的快速和尺寸控制生物合成具有抗菌活性

摘要 生物合成硒纳米粒子 (SeNPs) 由于其生物相容性和低毒性在生物医学应用中取得了相当大的重要性。在这项研究中,报道了通过印楝的水叶提取物快速和大小控制地生物合成 SeNP。SeNPs 是通过将亚硒酸钠盐的 Se+ 离子还原为元素硒来合成的。生物合成的 SeNP 本质上是晶体,呈球形,表面光滑。这些SeNPs的大小通过调节还原反应时间来控制。因此,在与初始浓度为 10 mM 的亚硒酸钠反应 5 和 10 分钟后,分别合成了尺寸范围为 142 至 168 nm 和 221 至 328 nm 的 SeNP。傅里叶变换红外 (FT-IR) 分析显示,SeNP 表面存在植物提取物分子,用作还原剂和封端分子。当通过 MTT 测定对 L929 细胞系进行体外测试时,发现这些生物合成的 SeNP 在性质上具有细胞相容性。此外,这些生物合成的 SeNP 对选定的革兰氏阳性和革兰氏阴性细菌菌株显示出有希望的抗菌活性。
更新日期:2020-04-01
down
wechat
bug