As a diverse and abundant class of endogenous RNAs, circular RNAs (circRNAs) participate in processes including cell proliferation and apoptosis. Nevertheless, few researchers have investigated the function of circRNAs in bovine muscle development. Based on existing sequencing data, we identified circINSR. The localization of circINSR in bovine myoblasts was investigated by fluorescence hybridization. Molecular and biochemical assays were used to confirm the role of circINSR in myoblast proliferation and the cell cycle. Mitochondrial membrane potential and annexin V-PE/7-AAD staining assays were performed to assess cell apoptosis. Additionally, interactions between circINSR, miR-34a, and target mRNAs were examined using bioinformatics, a luciferase assay, and RNA immunoprecipitation. We found that circINSR was highly expressed in embryonic muscle tissue. Overexpression of circINSR significantly promoted proliferation and reduced apoptosis of embryonic myoblasts. Our data suggested that circINSR may act as a sponge of miR-34a and could function through de-repression of target genes in muscle cells. This study proposes that circINSR may function as a regulator of embryonic muscle development. circINSR regulates cells proliferation and apoptosis through miR-34a-modulated Bcl-2 and CyclinE2 expression.

中文翻译：

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circINSR 通过海绵 miR-34a 促进胚胎成肌细胞增殖并减少细胞凋亡


作为一类多样化且丰富的内源性 RNA，环状 RNA (circRNA) 参与细胞增殖和凋亡等过程。然而，很少有研究人员研究 circRNA 在牛肌肉发育中的功能。根据现有的测序数据，我们鉴定了circINSR。通过荧光杂交研究了 circINSR 在牛成肌细胞中的定位。使用分子和生化测定来确认 circINSR 在成肌细胞增殖和细胞周期中的作用。进行线粒体膜电位和膜联蛋白 V-PE/7-AAD 染色测定来评估细胞凋亡。此外，还使用生物信息学、荧光素酶测定和 RNA 免疫沉淀检查了 circINSR、miR-34a 和靶 mRNA 之间的相互作用。我们发现circINSR在胚胎肌肉组织中高表达。 circINSR的过表达显着促进胚胎成肌细胞的增殖并减少细胞凋亡。我们的数据表明，circINSR 可能充当 miR-34a 的海绵，并可以通过肌肉细胞中靶基因的去抑制发挥作用。这项研究提出 circINSR 可能作为胚胎肌肉发育的调节剂。 circINSR 通过 miR-34a 调节的 Bcl-2 和 CyclinE2 表达来调节细胞增殖和凋亡。