BACKGROUND Streptococcus agalctiae (Group B Streptococcus, GBS) is a perinatal pathogen and a leading cause of neonatal infections worldwide. Serotype, sequence type, clonality, antibiotic resistance genes and surface protein profiles of GBS are scarce in Ethiopia, a reason that this study was planned to investigate. . METHODS Sixteen colonizing GBS isolates obtained from recto-vaginal swabs of pregnant women and body surfaces of newborns were further analyzed. Minimum inhibitory concentration (MIC) test, and whole genome sequence (WGS) methods were done for antibiotic susceptibility test, and molecular characterization of the isolates. RESULTS All the GBS isolates analyzed were belonged to four capsular serotypes: II, 11/16(68.8%), V, 3/16(18.8%), Ia and VI each with 1/16(6.3%) and five sequence type (ST-2, ST-10, ST-14, ST-569 and ST-933). Sequence type-10 was the most predominant ST followed by ST-569. The five STs were grouped into the four clonal complexes (CC - 1, CC-10, CC-19, and CC-23). Different surface proteins and pili families such as ALP1, ALPHA, ALP23, PI-1 / PI-2A1, PI-1 / PI-2B, and Srr1 were detected from WGS data. All isolates were found to be susceptible to the tested antibiotics except for tetracycline in MIC and WGS test methods used. Tetracycline resistant determinant genes such as TETM and TETL / TETM combination were identified. CONCLUSION Further studies on serotype and molecular epidemiology will provide a comprehensive data of the GBS capsular serotype and clones available in Ethiopia.

中文翻译：

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埃塞俄比亚西北部贡德尔大学综合专科医院从孕妇和新生儿分离出的无乳链球菌的分子特征。

背景技术无乳链球菌（B族链球菌，GBS）是围产期病原体，并且是全世界新生儿感染的主要原因。埃塞俄比亚缺乏GBS的血清型，序列类型，克隆性，抗生素抗性基因和表面蛋白谱，这是计划进行这项研究的原因。。方法进一步分析了从孕妇直肠阴道拭子和新生儿体表获得的16株定殖GBS分离株。进行了最小抑菌浓度（MIC）测试和全基因组序列（WGS）方法用于抗生素敏感性测试和分离株的分子表征。结果分析的所有GBS分离物均属于4种荚膜血清型：II，11/16（68.8％），V，3/16（18.8％），Ia和VI各自具有1/16（6.3％）和5种序列类型（ ST-2，ST-10，ST-14，ST-569和ST-933）。序列类型10是最主要的ST，其次是ST-569。将五个ST分为四个克隆复合体（CC-1，CC-10，CC-19和CC-23）。从WGS数据中检测到了不同的表面蛋白和菌毛家族，例如ALP1，ALPHA，ALP23，PI-1 / PI-2A1，PI-1 / PI-2B和Srr1。在所用的MIC和WGS测试方法中，除四环素外，所有分离株均对测试的抗生素敏感。鉴定了四环素抗性决定簇基因，例如TETM和TETL / TETM组合。结论对血清型和分子流行病学的进一步研究将提供埃塞俄比亚GBS荚膜血清型和克隆的全面数据。从WGS数据中检测到了不同的表面蛋白和菌毛家族，例如ALP1，ALPHA，ALP23，PI-1 / PI-2A1，PI-1 / PI-2B和Srr1。在所用的MIC和WGS测试方法中，除四环素外，所有分离株均对测试的抗生素敏感。鉴定了四环素抗性决定簇基因，例如TETM和TETL / TETM组合。结论对血清型和分子流行病学的进一步研究将提供埃塞俄比亚GBS荚膜血清型和可用克隆的全面数据。从WGS数据中检测到了不同的表面蛋白和菌毛家族，例如ALP1，ALPHA，ALP23，PI-1 / PI-2A1，PI-1 / PI-2B和Srr1。在所用的MIC和WGS测试方法中，除四环素外，所有分离株均对测试的抗生素敏感。鉴定了四环素抗性决定簇基因，例如TETM和TETL / TETM组合。结论对血清型和分子流行病学的进一步研究将提供埃塞俄比亚GBS荚膜血清型和克隆的全面数据。鉴定了四环素抗性决定簇基因，例如TETM和TETL / TETM组合。结论对血清型和分子流行病学的进一步研究将提供埃塞俄比亚GBS荚膜血清型和克隆的全面数据。鉴定了四环素抗性决定簇基因，例如TETM和TETL / TETM组合。结论对血清型和分子流行病学的进一步研究将提供埃塞俄比亚GBS荚膜血清型和克隆的全面数据。