Some sorghum cultivars are sensitive to organophosphate insecticides, which cause red to purple injury spots and, in severe cases, leaf death. Sensitivity to organophosphates is conditioned by a single locus on chromosome 5. We constructed a high‐density genetic map around the locus, termed osr, using DNA markers, and delimited osr to a 377‐kb region on the short arm of chromosome 5. Sequence analysis of this region predicted 19 gene candidates. Four of the candidates are homologous to a disease resistance NBS‐LRR gene. mRNA‐Seq analysis of gene expression and SNPs from two resistant cultivars (‘JN43’ and ‘Greenleaf’) and one sensitive cultivar (‘Nakei MS3B’) suggest that one or more members of this NBS‐LRR gene family are osr. Our results suggested that the recessive allele of osr results in sensitivity to OP insecticides.

中文翻译：

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高粱有机磷酸酯敏感反应候选基因的分子作图和鉴定

一些高粱品种对有机磷酸酯杀虫剂敏感，有机磷杀虫剂会引起红色至紫色的损伤点，严重时还会导致叶片死亡。对有机磷酸盐的敏感性取决于5号染色体上的单个基因座。我们使用DNA标记在该基因座周围建立了高密度遗传图谱，称为osr，并将osr限定在5号染色体短臂上的377kb区域。该区域的分析预测了19个基因候选者。其中四个候选基因与抗病性NBS-LRR基因同源。两个抗性品种（'JN43'和'Greenleaf'）和一个敏感品种（'Nakei MS3B'）基因表达和SNP的mRNA‐Seq分析表明该NBS‐LRR基因家族的一个或多个成员是osr。我们的结果表明，osr的隐性等位基因导致对OP杀虫剂的敏感性。