Titanium dioxide (TiO2) nanoparticle has good photo-/sono-catalytic features, the reunion of this particle in solution-phase generally limits the extensive biomedical application. In the present study, the aggregation of TiO2 nanoparticles was alleviated by facile fabrication under different pH conditions. A novel TiO2 nanocomposite was further synthesized by properly conjugation with trace amount of DVDMS sensitizer (named DFT). The characterization, sonoactivity, as well as the antibacterial efficiency were specially evaluated. The results showed that the sonochemical activity of DFT was greatly improved as compared with the simple surface modification of TiO2 (F-TiO2) and free DVDMS, regarding to the hydroxyl radicals and singlet oxygen yields using the same ultrasound exposure. Moreover, ultrasonic stimulation of DFT exhibited excellent bacterial eradication, with up to 92.41% of killing efficiency in S. aureus. The flow cytometry analysis indicated an increased intracellular ROS and membrane disturbance by combination of DFT and ultrasound. The findings suggest that the proper fabrication and DVDMS incorporation greatly improved the sonocatalytic process of TiO2, and the ultrasound based biomedical applications of DFT deserve future deep investigation.

中文翻译：

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用于增强抗菌活性的声活化TiO2-DVDMS纳米复合材料的制备。

二氧化钛（TiO2）纳米粒子具有良好的光/声催化特性，该粒子在溶液相中的团聚通常限制了广泛的生物医学应用。在本研究中，通过在不同pH条件下的简便制备来减轻TiO2纳米粒子的聚集。通过适当地与痕量DVDMS敏化剂（称为DFT）结合，可以进一步合成新型的TiO2纳米复合材料。特别评估了其表征，声音活性和抗菌效率。结果表明，与使用相同的超声暴露的羟基自由基和单重态氧收率相比，与简单的TiO2表面改性（F-TiO2）和游离DVDMS相比，DFT的声化学活性大大提高。此外，DFT的超声刺激具有出色的细菌根除效果，对金黄色葡萄球菌的杀灭效率高达92.41％。流式细胞仪分析表明DFT和超声的结合增加了细胞内ROS和膜紊乱。这些发现表明，适当的制造和DVDMS的结合大大改善了TiO2的声催化过程，DFT的基于超声的生物医学应用值得进一步深入研究。