Adenosine triphosphate (ATP) plays a crucial role in energy metabolism and extracellular purinergic signaling. A 3D bimetallic Au/Pt nanoflowers decorated ATP microelectrode biosensor prepared by facile and effective template-free electrodeposition was firstly reported, realizing local detection of cellular ATP secretion. The ATP biosensor was developed by co-immobilization of glucose oxidase and hexokinase, exhibiting long-term stability (79.39 ± 9.15% of its initial value remained after 14 days at 4 °C) and high selectivity with a limit of detection down to 2.5 μM (S/N = 3). The resulting ATP biosensor was then used for direct in situ monitoring of ATP secreted from living cells (PC12) with the stimulation of high K+ solutions. The obtained current was about 21.6 ± 3.4 nA (N = 6), corresponding to 12.2 ± 2.8 μM ATP released from cells, right in the micromolar range and consistent with the suggested levels. The 3D bimetallic Au/Pt nanoflowers possess excellent catalytic activity and large electroactive surface area, contributing to enzymatic activity preservation and long-term stability. This work provides a promising platform for long-time monitoring of other neurotransmitters and secretions in cellular glycolysis and apoptosis processes in the future.

中文翻译：

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3D双金属Au / Pt纳米花装饰了针型微电极，用于直接原位监测活细胞分泌的ATP。

三磷酸腺苷（ATP）在能量代谢和细胞外嘌呤能信号传导中起着至关重要的作用。首次报道了一种简便，有效的无模板电沉积制备的3D双金属金/铂纳米花修饰的ATP微电极生物传感器，实现了细胞ATP分泌的局部检测。ATP生物传感器是通过将葡萄糖氧化酶和己糖激酶共同固定化而开发的，具有长期稳定性（在4°C下放置14天后仍保持其初始值的79.39±9.15％）和高选择性，检测限低至2.5μM （S / N = 3）。然后将得到的ATP生物传感器用于在高K +溶液刺激下直接原位监测从活细胞（PC12）分泌的ATP。获得的电流约为21.6±3.4 nA（N = 6），相当于从细胞释放的12.2±2.8μMATP，在微摩尔范围内，并与建议的水平保持一致。3D双金属Au / Pt纳米花具有出色的催化活性和较大的电活性表面积，有助于保持酶活性和长期稳定性。这项工作为将来长期监测细胞糖酵解和凋亡过程中的其他神经递质和分泌物提供了一个有希望的平台。