Maintenance of the pluripotent state of mesenchymal stem cells (MSCs) during in vitro expansion is an important factor for the successful proliferation of MSCs possessing high differentiation capacity. However, the differentiation potential of MSCs can easily be lost during in vitro expansion, particularly at late passages. Reactive oxygen species (ROS) are signaling molecules that help to maintain MSC function; however, excessive ROS generation can induce senescence and impair both the differentiation capacity and proliferation of MSCs. In this study, we have designed an amphiphilic block copolymer (redox copolymer), which possesses ROS scavenging capacity in the hydrophobic site. When this redox copolymer was coated on cell culture dishes coupled with human E‐cadherin chimeric antibody (hE‐cad‐Fc), it had an antioxidative effect on cultured MSCs. We also confirmed that the redox polymer construct poly(ethylene glycol) tethered chain on the surface prevented nonspecific cell binding, whereas the co‐immobilized surface allowed high adhesion of E‐cadherin‐positive MSCs. Interestingly, the intracellular ROS level was significantly decreased by the prepared cell culture dish, despite ROS being scavenged only on the surface of the dish, on the cell exterior. Consequently, the cultured MSCs retained high expression levels of pluripotency‐associated genes, including SOX2.

中文翻译：

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抗氧化细胞培养皿抑制细胞内活性氧的积累并调节间充质干细胞中多能性相关基因的表达。

在体外扩增过程中维持间充质干细胞 (MSCs) 的多能状态是具有高分化能力的 MSCs 成功增殖的重要因素。然而，MSCs 的分化潜能在体外扩增过程中很容易丧失，特别是在后期传代时。活性氧 (ROS) 是有助于维持 MSC 功能的信号分子；然而，过量的 ROS 产生会诱导衰老并损害 MSCs 的分化能力和增殖能力。在这项研究中，我们设计了一种两亲性嵌段共聚物（氧化还原共聚物），它在疏水部位具有 ROS 清除能力。当这种氧化还原共聚物与人 E-cadherin 嵌合抗体 (hE-cad-Fc) 一起涂覆在细胞培养皿上时，它对培养的 MSC 具有抗氧化作用。我们还证实，表面上的氧化还原聚合物构建聚（乙二醇）系链阻止了非特异性细胞结合，而共同固定的表面允许 E-钙粘蛋白阳性 MSC 的高粘附。有趣的是，制备的细胞培养皿显着降低了细胞内 ROS 水平，尽管 ROS 仅在培养皿表面、细胞外部被清除。因此，培养的 MSC 保留了多能性相关基因的高表达水平，包括 SOX2。尽管 ROS 仅在培养皿表面、细胞外部被清除。因此，培养的 MSC 保留了多能性相关基因的高表达水平，包括 SOX2。尽管 ROS 仅在培养皿表面、细胞外部被清除。因此，培养的 MSC 保留了多能性相关基因的高表达水平，包括 SOX2。