Enzymatic hydrolysis processes can change the physical characteristics of nanocellulose derived from Kraft pulp. Among these attributes are its crystallinity index and dimensions. In this study, we determined the optimal conditions under which nanocellulose could be produced enzymatically with the greatest increase of the crystallinity index relative to its initial state. Application of Central Composite Rotatable Design statistical analysis to the experiments was employed to direct an increase the crystallinity index in 10% at the 24‐H hydrolysis time. Upon establishment of ideal levels of starting material and enzyme, reactions were carried out at hydrolysis times of 24, 48, and 72 H under these ideal parameters. The effectiveness of deagglomeration was demonstrated by measuring the hydrodynamic diameter of the particles by dynamic light scattering. Scanning electron microscopy was performed on four samples, the original material, kraft pulp, and hydrolyzed biomaterials at 72 H in the ideal parameters. The hydrolyzed material with the best statistical data, revealing a fiber diameter of 180 nm, disclosing to be biomaterial with nanocellulose dimensions.

中文翻译：

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经牛皮纸浆通过受控酶水解生产的纳米纤维素的结晶度指标的统计分析。

酶促水解过程可以改变源自牛皮纸浆的纳米纤维素的物理特性。这些属性包括其结晶度指数和尺寸。在这项研究中，我们确定了最佳的条件，在该条件下可以酶法生产纳米纤维素，相对于其初始状态，其结晶度指数最大。在实验中应用中央复合可旋转设计统计分析，以指示在24 H水解时间将结晶度指数提高10％。在确定起始原料和酶的理想水平后，在这些理想参数下于24、48和72 H的水解时间进行反应。通过用动态光散射测量颗粒的流体动力学直径证明了解团聚的有效性。在理想参数下，在72 H下对四个样品（原始材料，牛皮纸浆和水解的生物材料）进行了扫描电子显微镜检查。具有最佳统计数据的水解材料，揭示的纤维直径为180 nm，公开了具有纳米纤维素尺寸的生物材料。