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Continuous flow reactor based with an immobilized biocatalyst for the continuous enzymatic transesterification of crude coconut oil.
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-01-12 , DOI: 10.1002/bab.1885 Juliana L Santana 1 , Juliana M Oliveira 1 , Jamily S Nascimento 1 , Silvana Mattedi 2 , Laiza C Krause 3 , Lisiane S Freitas 4 , Eliane B Cavalcanti 1 , Matheus M Pereira 5 , Álvaro S Lima 1 , Cleide M F Soares 1
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-01-12 , DOI: 10.1002/bab.1885 Juliana L Santana 1 , Juliana M Oliveira 1 , Jamily S Nascimento 1 , Silvana Mattedi 2 , Laiza C Krause 3 , Lisiane S Freitas 4 , Eliane B Cavalcanti 1 , Matheus M Pereira 5 , Álvaro S Lima 1 , Cleide M F Soares 1
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Here, we have assessed the use of one packed bed or two packed bed reactors in series in which Burkholderia cepacia lipase (BCL) was immobilized on protic ionic liquid (PIL)‐modified silica and used as a biocatalyst for the transesterification of crude coconut oil. Reaction parameters including volumetric flow, temperature, and molar ratio were evaluated. The conversion of transesterification reaction products (ethyl esters) was determined using gas chromatography and the quantities of intermediate products (diglyceride and monoglyceride [MG]) were assessed using high‐performance liquid chromatography. Packed bed reactors in series produced ethyl esters with the greatest efficiency, achieving 65.27% conversion after 96 H at a volumetric flow rate of 0.50 mL Min−1 at 40 °C and a 1:9 molar ratio of oil to ethanol. Further, within the first 24 H of the reaction, increased MG (54.5%) production was observed. Molecular docking analyses were performed to evaluate the catalytic step of coconut oil transesterification in the presence of BCL. Molecular docking analysis showed that triglycerides have a higher affinity energy (−5.7 kcal mol−1) than the smallest MG (−6.0 kcal mol−1), therefore, BCL catalyzes the conversion of triglycerides rather than MG, which is consistent with experimental results.
中文翻译：
基于固定化生物催化剂的连续流反应器，用于椰子油的连续酶促酯交换。
在这里，我们评估了串联使用一个填充床或两个填充床反应器的情况，其中伯克霍尔德菌洋葱脂肪酶（BCL）固定在质子离子液体（PIL）改性的二氧化硅上，并用作生椰子油酯交换的生物催化剂。评价了包括体积流量，温度和摩尔比的反应参数。使用气相色谱法测定酯交换反应产物（乙酯）的转化率，并使用高效液相色谱法评估中间产物（甘油二酯和甘油单酯[MG]）的量。串联的填充床反应器以最高的效率生产乙酯，在96 H后，体积流量为0.50 mL Min -1时，转化率为65.27％在40°C和油与乙醇的摩尔比为1：9的情况下。此外，在反应的前24小时内，观察到增加的MG（54.5％）产生。进行分子对接分析以评估在BCL存在下椰子油酯交换反应的催化步骤。分子对接分析表明，甘油三酸酯比最小的MG（-6.0 kcal mol -1）具有更高的亲和能（-5.7 kcal mol -1），因此，BCL催化甘油三酸酯的转化而不是MG，这与实验结果一致。
更新日期：2020-01-12
中文翻译：
基于固定化生物催化剂的连续流反应器，用于椰子油的连续酶促酯交换。
在这里，我们评估了串联使用一个填充床或两个填充床反应器的情况，其中伯克霍尔德菌洋葱脂肪酶（BCL）固定在质子离子液体（PIL）改性的二氧化硅上，并用作生椰子油酯交换的生物催化剂。评价了包括体积流量，温度和摩尔比的反应参数。使用气相色谱法测定酯交换反应产物（乙酯）的转化率，并使用高效液相色谱法评估中间产物（甘油二酯和甘油单酯[MG]）的量。串联的填充床反应器以最高的效率生产乙酯，在96 H后，体积流量为0.50 mL Min -1时，转化率为65.27％在40°C和油与乙醇的摩尔比为1：9的情况下。此外，在反应的前24小时内，观察到增加的MG（54.5％）产生。进行分子对接分析以评估在BCL存在下椰子油酯交换反应的催化步骤。分子对接分析表明，甘油三酸酯比最小的MG（-6.0 kcal mol -1）具有更高的亲和能（-5.7 kcal mol -1），因此，BCL催化甘油三酸酯的转化而不是MG，这与实验结果一致。
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