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Microwave‐assisted acid hydrolysis for whole‐bone proteomics and paleoproteomics
Rapid Communications in Mass Spectrometry ( IF 1.8 ) Pub Date : 2020-01-10 , DOI: 10.1002/rcm.8568
Caitlin Colleary 1 , Nicole C. Little 1 , Timothy P. Cleland 1
Affiliation  

Whole‐bone proteomic analyses rely on lengthy sample preparation including demineralization and digestion to break bone down into peptides to recover using mass spectrometry. However, microwave‐assisted acid hydrolysis, a technique used in proteomic analyses of other soft tissues and cells, will combine both demineralization and digestion and only take minutes.

中文翻译:

微波辅助酸水解用于全骨蛋白质组学和古生物学

全骨蛋白质组学分析需要冗长的样品准备工作,包括去矿物质和消化,以将骨骼分解成肽,然后使用质谱法进行回收。但是,微波辅助酸水解是一种用于其他软组织和细胞的蛋白质组学分析的技术,它将脱盐和消化结合在一起,并且只需几分钟。
更新日期:2020-01-13
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