BACKGROUND Liver disorders may be associated with normal pulmonary hemodynamic, hepatopulmonary syndrome (HPS), or portopulmonary hypertension (POPH). In this study, we aimed to investigate the effect of the severity of liver dysfunctions on blood-borne variables, and pulmonary hemodynamic during repeated ventilation with hyperoxic and hypoxic gases. METHODS Female Sprague Dawley rats were assigned into four groups of Sham (n = 7), portal vein ligation (PPVL, n = 7), common bile duct ligation (CBDL, n = 7), and combination of them (CBDL+ PPVL, n = 7). Twenty-eight days later, right ventricular systolic pressure (RVSP) and systemic blood pressure were recorded in anesthetized animals subjected to repeated maneuvers of hyperoxia (O2 50%) and hypoxia (O2 10%). Besides, we assessed blood parameters and liver histology. RESULTS Liver histology score, liver enzymes, WBC and plasma malondialdehyde in the CBDL+PPVL group were higher than those in the CBDL group. Also, the plasma platelet level in the CBDL+PPVL group was lower than those in the other groups. On the other hand, the serum estradiol in the CBDL group was higher than that in the CBDL+PPVL group. All the above parameters in the PPVL group were similar to those in the Sham group. During ventilation with hyperoxia gas, RVSP in the CBDL+PPVL group was higher than the ones in the other groups, and in the CBDL group, it was more than those in the PPVL and Sham groups. Hypoxic pulmonary vasoconstriction (HPV) was not detected in both CBDL+PPVL and CBDL groups, whereas, it retained in the PPVL group. CONCLUSION Severe liver damage increases RVSP in the CBDL+PPVL group linked to the high level of ROS, low levels of serum estradiol and platelets or a combination of them. Furthermore, the high RVSP at the noted group could present a reliable animal model for POPH in female rats.

中文翻译：

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肝损伤对麻醉大鼠血源性变量和对低氧和高氧的肺血流动力学的影响。

背景技术肝脏疾病可能与正常的肺血流动力学，肝肺综合征（HPS）或肺门高压（POPH）有关。在这项研究中，我们旨在研究在高氧和低氧气体反复通气期间，肝功能障碍严重程度对血源性变量和肺血流动力学的影响。方法将雌性Sprague Dawley大鼠分为四组：假手术（n = 7），门静脉结扎（PPVL，n = 7），胆总管结扎（CBDL，n = 7）以及它们的组合（CBDL + PPVL，n = 7）。二十八天后，在经历过高氧（O2 50％）和缺氧（O2 10％）反复操作的麻醉动物中记录了右心室收缩压（RVSP）和全身血压。此外，我们评估了血液参数和肝脏组织学。结果肝组织学评分，CBDL + PPVL组的肝酶，WBC和血浆丙二醛均高于CBDL组。另外，CBDL + PPVL组的血浆血小板水平低于其他组。另一方面，CBDL组的血清雌二醇高于CBDL + PPVL组。PPVL组中的所有上述参数与Sham组中的相似。在高氧气体通气期间，CBDL + PPVL组的RVSP高于其他组，而CBDL组的RVSP高于PPVL和Sham组。CBDL + PPVL和CBDL组均未检测到低氧性肺血管收缩（HPV），而PPVL组中仍存在。结论CBDL + PPVL组严重肝损害会增加RVSP，这与ROS水平高有关，血清雌二醇和血小板水平低或二者结合。此外，在上述一组中，高RVSP可以为雌性大鼠提供可靠的POPH动物模型。