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Characterization of a novel cotton MYB gene, GhMYB108-like responsive to abiotic stresses.
Molecular Biology Reports ( IF 2.6 ) Pub Date : 2020-01-13 , DOI: 10.1007/s11033-020-05244-6
Abid Ullah 1, 2 , Muhammad Tahir Ul Qamar 1, 3 , Mohammad Nisar 2 , Ali Hazrat 2 , Gul Rahim 2 , Aamir Hamid Khan 1 , Kashif Hayat 4 , Saeed Ahmed 5 , Waqar Ali 6 , Aziz Khan 7 , Xiyan Yang 1
Affiliation  

Transcriptional factors are the major regulators of plant signaling pathways in response to environmental stresses i.e., drought, salinity and cold. Hereby, the GhMYB108-like was characterized to determine whether it regulate these stresses. The GhMYB108-like cDNA consisted of 1107 base pairs (bp) with 807 open reading frame encoded a protein of 268 amino acids. Its isoelectric point and molecular weight are 5.51 and 30.3 kDa respectively. Phylogenetic analysis and online databases revealed that GhMYB108-like proteins are closely related with the Arabidopsis thaliana MYB2. Important cis-elements were detected in the promotor region of GhMYB108-like responding to stresses and phytohormones. The 3D structure of GhMYB108-like protein has been predicted. In addition, various physico-chemical properties of GhMYB108-like have been determined. Subcellular localization confirmed that GhMYB108-like are nuclear localized protein. Quantitative expression analysis showed that polyethylene glycol and salt treatments significantly induced the expression of GhMYB108-like. Overall, our findings suggest that GhMYB108-like is an important gene that would plays important regulatory role in response to drought and salt stresses.

中文翻译:

新型棉花MYB基因GhMYB108-样对非生物胁迫的响应。

转录因子是响应环境胁迫(例如干旱,盐碱和寒冷)的植物信号传导途径的主要调节因子。因此,表征了GhMYB108样以确定其是否调节这些应激。GhMYB108样cDNA由1107个碱基对(bp)组成,具有807个开放阅读框,编码268个氨基酸。其等电点和分子量分别为5.51和30.3 kDa。系统发育分析和在线数据库显示,GhMYB108样蛋白与拟南芥MYB2密切相关。在应激和植物激素响应的GhMYB108样启动子区域检测到重要的顺式元素。已经预测了GhMYB108样蛋白的3D结构。另外,已经确定了GhMYB108样的各种理化性质。亚细胞定位证实GhMYB108样是核定位蛋白。定量表达分析表明,聚乙二醇和盐处理可显着诱导GhMYB108-like的表达。总体而言,我们的发现表明,GhMYB108-like是一个重要的基因,在应对干旱和盐胁迫时将发挥重要的调节作用。
更新日期:2020-01-13
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