Abstract Mesoporous bioactive glass demonstrated valuable biomedical applications especially in the field of bone regeneration and drug delivery. For the treatment of cancer by chemotherapy, controlled drug delivery system is one of the significant methods. In this work, silver oxide doped mesoporous bioactive glass nanoparticles (Ag2O-MBG NPs) were developed for the controlled release of doxorubicin as a model drug. Ag2O-MBG NPs were prepared by using the sol-gel method and employing pluronic 123 as an internal template. The prepared Ag2O-MBG NPs were characterized by energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM) and transmission electron microscopy (TEM) for composition, shape, morphology, and size. Surface area and pore size were determined by the Brunauer-Emmett-Teller (BET) and Barrett-Joyner-Halenda (BJH) method respectively. When immersed in simulated body fluid (SBF), hydroxycarbonate apatite (HCAp) formation was demonstrated as established by Fourier Transform Infrared (FTIR) spectroscopy and X-ray diffraction (XRD). The as-synthesized Ag2O-MBG NPs did not show any detrimental effects during MTT assay and in vivo tissue histopathology. Doxorubicin (DOX) was encapsulated with an efficiency of 84% and its release was observed to be affected by the drug loading concentration (0.2–1.0 mg/mL) and pH (6.4–8.4) of the release media. DOX release was of 93% was witnessed approximately for two weeks at a slight acidic pH of 6.4. At 11.88 μg/mL of DOX-Ag2O-MBG NPs, notable inhibitory effects on the viability of the MG-63 osteosarcoma cancer cells were observed. These features proved that the Ag2O-MBG NPs system is effective for bone tissue regeneration and bone cancer treatment.

中文翻译：

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成骨氧化银掺杂介孔生物活性玻璃用于控制释放阿霉素对骨癌细胞系 (MG-63)：体外和体内细胞毒性评估

摘要 介孔生物活性玻璃展示了有价值的生物医学应用，特别是在骨再生和药物输送领域。对于通过化学疗法治疗癌症，受控给药系统是重要的方法之一。在这项工作中，氧化银掺杂的介孔生物活性玻璃纳米粒子 (Ag2O-MBG NPs) 被开发用于控制释放阿霉素作为模型药物。Ag2O-MBG NPs 是通过使用溶胶-凝胶方法和使用 pluronic 123 作为内部模板制备的。通过能量色散 X 射线光谱 (EDX)、扫描电子显微镜 (SEM) 和透射电子显微镜 (TEM) 对制备的 Ag2O-MBG NPs 的成分、形状、形态和尺寸进行表征。表面积和孔径分别通过 Brunauer-Emmett-Teller (BET) 和 Barrett-Joyner-Halenda (BJH) 方法测定。当浸入模拟体液 (SBF) 中时，通过傅立叶变换红外 (FTIR) 光谱和 X 射线衍射 (XRD) 证实了羟基碳酸盐磷灰石 (HCAP) 的形成。在 MTT 测定和体内组织病理学期间，合成的 Ag2O-MBG NPs 没有显示任何有害影响。多柔比星 (DOX) 的封装效率为 84%，观察到其释放受释放介质的载药浓度 (0.2–1.0 mg/mL) 和 pH (6.4–8.4) 影响。在 6.4 的弱酸性 pH 值下，大约两周内见证了 93% 的 DOX 释放。在 11.88 μg/mL 的 DOX-Ag2O-MBG NPs 下，观察到对 MG-63 骨肉瘤癌细胞活力的显着抑制作用。这些特征证明 Ag2O-MBG NPs 系统对骨组织再生和骨癌治疗有效。