Detection of HER2+ Breast Cancer Cells using Bioinspired DNA-Based Signal Amplification.,ChemMedChem

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Detection of HER2+ Breast Cancer Cells using Bioinspired DNA-Based Signal Amplification.
ChemMedChem ( IF 3.6 ) Pub Date : 2020-01-16 , DOI: 10.1002/cmdc.201900697
Sarah D Rafiee ₁ , Samet Kocabey ₁ , Michael Mayer ₂ , Jonathan List _{2,

3} , Curzio Rüegg ₁

Affiliation

Circulating tumor cells (CTC) are promising biomarkers for metastatic cancer detection and monitoring progression. However, detection of CTCs remains challenging due to their low frequency and heterogeneity. Herein, we report a bioinspired approach to detect individual cancer cells, based on a signal amplification cascade using a programmable DNA hybridization chain reaction (HCR) circuit. We applied this approach to detect HER2+ cancer cells using the anti-HER2 antibody (trastuzumab) coupled to initiator DNA eliciting a HCR cascade that leads to a fluorescent signal at the cell surface. At 4 °C, this HCR detection scheme resulted in highly efficient, specific and sensitive signal amplification of the DNA hairpins specifically on the membrane of the HER2+ cells in a background of HER2- cells and peripheral blood leukocytes, which remained almost non-fluorescent. The results indicate that this system offers a new strategy that may be further developed toward an in vitro diagnostic platform for the sensitive and efficient detection of CTC.

中文翻译：

使用基于生物启发的DNA信号扩增检测HER2 +乳腺癌细胞。

循环肿瘤细胞（CTC）是用于转移性癌症检测和监测进展的有前途的生物标志物。但是，由于CTC的频率低和异质性，其检测仍然具有挑战性。在这里，我们报告了一种基于生物的方法来检测单个癌细胞，该方法基于使用可编程DNA杂交链反应（HCR）电路的信号放大级联。我们将这种方法用于抗HER2抗体（曲妥珠单抗）与引发DNA引发HCR级联反应的引发剂DNA偶联的抗HER2抗体（曲妥珠单抗），从而检测HER2 +癌细胞。在4°C下，此HCR检测方案可在HER2细胞和外周血白细胞的背景下，对HER2 +细胞膜上的DNA发夹进行高效，特异性和灵敏的信号放大，几乎保持无荧光。结果表明，该系统提供了一种新的策略，可以进一步开发出一种体外诊断平台，用于灵敏和有效地检测CTC。

更新日期：2020-01-16

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