Ocular toxoplasmosis associated with up-regulation of miR-155-5p/miR-29c-3p and down-regulation of miR-21-5p/miR-125b-5p,Cytokine

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Ocular toxoplasmosis associated with up-regulation of miR-155-5p/miR-29c-3p and down-regulation of miR-21-5p/miR-125b-5p
Cytokine ( IF 3.7 ) Pub Date : 2020-03-01 , DOI: 10.1016/j.cyto.2020.154990
Cristina Silva Meira-Strejevitch ₁ , Ingrid de Siqueira Pereira ₁ , Daise Damaris Carnietto Hippólito ₁ , Marta Marques Maia ₁ , Allecineia Bispo Cruz ₁ , Ricardo Gava ₁ , Cinara Cássia Brandão de Mattos ₂ , Fábio Batista Frederico ₃ , Rubens Camargo Siqueira ₃ , Luiz Carlos Mattos ₂ , Vera Lucia Pereira-Chioccola ₁

Affiliation

Ocular toxoplasmosis (OT) is one of the most common manifestations of Toxoplasma gondii infection and can be related with congenital or acquired infections. OT cause posterior uveitis that cause serious sequelae as complete loss of vision. microRNAs (miRNAs) are small non-coding RNAs, which have regulatory roles in cells by silencing messenger RNA. This study evaluated gene expression of miR-155-5p, miR-146a-5p, miR-21-5p, miR-29c-3p and miR-125b-5p in plasma of 51 patients with ocular toxoplasmosis (OT Group), 26 individuals with asymptomatic toxoplasmosis (AT Group), and 25 healthy individuals seronegative for toxoplasmosis (NC Group). Peripherical blood samples were collected in tube with EDTA for plasma isolation, laboratorial diagnosis for toxoplasmosis and RNA extraction. miRNA expression of each sample was performed by qPCR and values were expressed in Relative Quantification (RQ). Results showed that miR-155-5p and miR-29c-3p were up-expressed in OT patients than AT individuals. On the other hand, miR-21-5p and miR-125b-5p were down-expressed in OT patients. Differences were statistically significant. miR-146a-5p expression was similar in OT patients and AT individuals, without significant difference. In addition, comparative analysis for miRNA levels between AT and OT groups confirms these results. So far, this is the first study to evaluate circulating miRNA levels in ocular toxoplasmosis. These findings may contribute to further studies evaluating the exact role of these miRNAs in the course of infection, which may help in understanding the complex parasite-host interaction and future use in diagnosis, prognosis and therapeutic control in ocular toxoplasmosis.

中文翻译：

与 miR-155-5p/miR-29c-3p 上调和 miR-21-5p/miR-125b-5p 下调相关的眼部弓形虫病

眼弓形虫病 (OT) 是弓形虫感染最常见的表现之一，可能与先天性或后天性感染有关。OT 引起后葡萄膜炎，导致严重的后遗症，如完全丧失视力。microRNA (miRNA) 是小的非编码 RNA，通过沉默信使 RNA 在细胞中发挥调节作用。本研究评估了 51 名眼弓形体病患者（OT 组）26 人血浆中 miR-155-5p、miR-146a-5p、miR-21-5p、miR-29c-3p 和 miR-125b-5p 的基因表达无症状弓形体病（AT 组）和 25 名弓形体病血清阴性的健康个体（NC 组）。外周血样本用 EDTA 收集在管中，用于血浆分离、弓形体病的实验室诊断和 RNA 提取。每个样品的 miRNA 表达通过 qPCR 进行，值以相对定量 (RQ) 表示。结果显示 miR-155-5p 和 miR-29c-3p 在 OT 患者中的表达高于 AT 个体。另一方面，miR-21-5p 和 miR-125b-5p 在 OT 患者中下调。差异具有统计学意义。miR-146a-5p 表达在 OT 患者和 AT 个体中相似，无显着差异。此外，AT 和 OT 组之间 miRNA 水平的比较分析证实了这些结果。到目前为止，这是第一项评估眼部弓形体病循环 miRNA 水平的研究。这些发现可能有助于进一步研究评估这些 miRNA 在感染过程中的确切作用，这可能有助于了解复杂的寄生虫 - 宿主相互作用和未来在诊断中的用途，

更新日期：2020-03-01

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