Abstract Antimicrobial resistance has gradually become a serious problem threatening public health and food safety throughout the world. Biofilm is one of the important factors affecting the antimicrobial resistance of bacteria. Staphylococcus aureus usually has strong biofilm formation ability, and it is widely found in animal-based food. The purpose of this study is to determine the prevalence, antimicrobial resistance and biofilm formation of S. aureus in animal-based food. Total 959 samples representing eight types of animal-based foods were collected from randomly selected locations (21 supermarkets and 18 wet markets) throughout the Shanghai city. The overall isolation rate of S. aureus was 17.2% (165/959). For each food category, the isolation rate was 21.8% for chicken (45/206), 21.5% for pork (71/331), 15.2% for beef (16/105), 13.8% for duck (9/65), 12.1% for aquatic products (17/141), 8.6% for egg (5/58), and 7.1% for lamb (2/28), respectively. No isolate was found from pasteurized milk (n = 25). Antimicrobial susceptibility test showed that among all the S. aureus isolates, 90.3% were resistant to at least one antimicrobial, 39.4% were multi-drug resistant, and 23 isolates were methicillin-resistant S. aureus (MRSA). Comparing the resistance rates to different antimicrobials, S. aureus had the highest resistance rate to penicillin, up to 82.4% (136/165); followed by erythromycin (57.6%, 95/165) and tetracycline (27.9%, 46/165). All isolates were sensitive to vancomycin. With the microtiter plate and crystal violet staining assay, 64.8% of all the 165 isolates had strong biofilm formation ability and 20.0% were moderate producers. Remarkably, significant difference was found in biofilm formation ability between those isolates from supermarkets and wet markets (p

中文翻译：

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上海市动物性食品中生物膜形成能力强的耐多药金黄色葡萄球菌的流行情况

摘要 抗生素耐药性已逐渐成为威胁全球公共卫生和食品安全的严重问题。生物膜是影响细菌耐药性的重要因素之一。金黄色葡萄球菌通常具有较强的生物膜形成能力，广泛存在于动物性食品中。本研究的目的是确定动物性食品中金黄色葡萄球菌的流行率、抗菌素耐药性和生物膜形成。从整个上海市随机选择的地点（21 个超市和 18 个湿货市场）收集了代表 8 种动物性食品的 959 个样品。金黄色葡萄球菌的总分离率为 17.2% (165/959)。对于每个食品类别，鸡肉（45/206）的隔离率为21.8％，猪肉（71/331）为21.5％，牛肉（16/105）为15.2％，13。鸭肉8%（9/65），水产品12.1%（17/141），鸡蛋8.6%（5/58），羊肉7.1%（2/28）。没有从巴氏杀菌奶中发现分离物（n = 25）。抗菌药物药敏试验表明，在所有金黄色葡萄球菌分离株中，90.3%对至少一种抗生素耐药，39.4%为多重耐药，23株为耐甲氧西林金黄色葡萄球菌（MRSA）。比较不同抗菌药物的耐药率，金黄色葡萄球菌对青霉素的耐药率最高，高达82.4%（136/165）；其次是红霉素（57.6%，95/165）和四环素（27.9%，46/165）。所有分离株均对万古霉素敏感。通过微量滴定板和结晶紫染色测定，165 株分离株中有 64.8% 具有较强的生物膜形成能力，20.0% 为中等生产者。值得注意的是，