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Biofilm formation of clinically important microorganisms on 2D and 3D poly (methyl methacrylate) substrates: A surface-enhanced Raman scattering study.
Colloids and Surfaces B: Biointerfaces ( IF 5.4 ) Pub Date : 2020-01-13 , DOI: 10.1016/j.colsurfb.2019.110765 Seda Keleştemur 1 , Zehra Çobandede 1 , Mustafa Çulha 1
Colloids and Surfaces B: Biointerfaces ( IF 5.4 ) Pub Date : 2020-01-13 , DOI: 10.1016/j.colsurfb.2019.110765 Seda Keleştemur 1 , Zehra Çobandede 1 , Mustafa Çulha 1
Affiliation
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Clinically relevant microorganisms threaten patient's health often through biofilm formation on polymeric medical devices and implants. Poly (methyl methacrylate) is a commonly used polymer in medical implants and dental devices. In this study, biofilm characteristics of model microorganisms, Pseudomonas aeruginosa, Staphylococcus epidermidis and Candida albicans, were investigated at molecular level on 2-dimensional (2D) and 3-dimensional (3D) PMMA substrates to understand the influence of surface structures on biofilm formation and also to demonstrate the discrimination of microorganisms according to their metabolic activities by utilizing surface-enhanced Raman scattering (SERS). It was found that the fibrous 3D structure enhanced the assembly of microorganisms and enriched the biofilm structure while smooth polymeric surface decreased the biofilm formation rate and variety of biofilm content. Among the studied microorganisms, Pseudomonas aeruginosa and Candida albicans had a higher tendency to form biofilm on both 2D and 3D PMMA substrates. Although Staphlylococcus epidermidis showed slow adaption on PMMA surfaces, the 3D porous surfaces increased its biofilm formation rate significantly compared to 2D surface.
中文翻译：
在2D和3D聚（甲基丙烯酸甲酯）基质上形成临床上重要的微生物的生物膜形成：表面增强拉曼散射研究。
临床相关的微生物通常通过在聚合医疗设备和植入物上形成生物膜来威胁患者的健康。聚（甲基丙烯酸甲酯）是医疗植入物和牙科设备中常用的聚合物。在这项研究中，模型分子，铜绿假单胞菌，表皮葡萄球菌和白色念珠菌的生物膜特性在二维（2D）和3维（3D）PMMA底物上进行了分子水平研究，以了解表面结构对生物膜形成的影响。并通过利用表面增强拉曼散射（SERS）证明了根据微生物的代谢活性对微生物的区分。发现纤维状3D结构增强了微生物的组装并丰富了生物膜的结构，而光滑的聚合物表面降低了生物膜的形成速率和生物膜含量的变化。在研究的微生物中，铜绿假单胞菌和白色念珠菌在2D和3D PMMA底物上都有较高的形成生物膜的趋势。尽管表皮葡萄球菌在PMMA表面上显示缓慢的适应性，但与2D表面相比，3D多孔表面显着提高了其生物膜形成速率。
更新日期：2020-01-13
中文翻译：
在2D和3D聚（甲基丙烯酸甲酯）基质上形成临床上重要的微生物的生物膜形成：表面增强拉曼散射研究。
临床相关的微生物通常通过在聚合医疗设备和植入物上形成生物膜来威胁患者的健康。聚（甲基丙烯酸甲酯）是医疗植入物和牙科设备中常用的聚合物。在这项研究中，模型分子，铜绿假单胞菌，表皮葡萄球菌和白色念珠菌的生物膜特性在二维（2D）和3维（3D）PMMA底物上进行了分子水平研究，以了解表面结构对生物膜形成的影响。并通过利用表面增强拉曼散射（SERS）证明了根据微生物的代谢活性对微生物的区分。发现纤维状3D结构增强了微生物的组装并丰富了生物膜的结构，而光滑的聚合物表面降低了生物膜的形成速率和生物膜含量的变化。在研究的微生物中，铜绿假单胞菌和白色念珠菌在2D和3D PMMA底物上都有较高的形成生物膜的趋势。尽管表皮葡萄球菌在PMMA表面上显示缓慢的适应性，但与2D表面相比，3D多孔表面显着提高了其生物膜形成速率。
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