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FRET probe for selective and sensitive detection of vitamin A by cadmium free quantum dots (ZnS).
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy ( IF 4.3 ) Pub Date : 2020-01-11 , DOI: 10.1016/j.saa.2020.118062
Sara Safari 1 , Ahmad Amiri 2 , Alireza Badiei 2
Affiliation  

Vitamin A as a powerful antioxidant plays an important role in human body functions including bone remodeling regulation, healthy immune system and cell growth reproduction. An accurate determination of vitamin A is taken into consideration because of its importance for human health. In this paper, we reported a fluorescence resonance energy transfer (FRET) probe, MPS-capped ZnS QDs, for sensitive and selective detection of vitamin A. The colloidal MPS-capped ZnS QDs were prepared from Zinc acetate and sodium sulfide by employing 3-mercaptopropyltrimethoxysilane (MPS) molecules as the stabilizer or capping agent at the pH condition of 10. The synthesized MPS-capped ZnS QDs were characterized by means of FT-IR, UV-Vis, DLS, and TEM techniques. The sensing behavior of MPS-capped ZnS QDs for selective and sensitive detection of vitamin A, vitamin B2, vitamin B6, vitamin E, vitamin K, vitamin H, vitamin D3 and vitamin C was investigated using fluorescence spectroscopy. The detection mechanism involves photoinduced charge transfer from the surface of ZnS QDs to Vitamin resulting in the fluorescence quenching of ZnS QDs followed by nonradiative fluorescence resonance energy transfer. An excellent selectivity was observed for vitamin A versus other tested species. A linear relationship was observed between the fluorescence intensity of MPS-capped ZnS QDs and the concentration of vitamin A in the range of 3.33-36.66 μM with detection limit of 1.062 μM.

中文翻译:

FRET探针,用于通过无镉量子点(ZnS)选择性和灵敏地检测维生素A。

维生素A作为强大的抗氧化剂,在人体功能(包括骨骼重塑调节,健康的免疫系统和细胞生长繁殖)中起着重要作用。考虑到维生素A的准确测定,因为它对人体健康至关重要。在本文中,我们报道了一种荧光共振能量转移(FRET)探针,用MPS封端的ZnS QD灵敏和选择性地检测维生素A。用乙酸锌和硫化钠通过胶体MPS封固的ZnS QD的制备是采用3-巯基丙基三甲氧基硅烷(MPS)分子在10的pH条件下作为稳定剂或封端剂。通过FT-IR,UV-Vis,DLS和TEM技术对合成的MPS封端的ZnS QD进行表征。MPS覆盖的ZnS QD的传感行为,用于选择性和灵敏地检测维生素A,维生素B2,使用荧光光谱法研究了维生素B6,维生素E,维生素K,维生素H,维生素D3和维生素C。检测机制涉及从ZnS QDs到维生素的光诱导电荷转移,导致ZnS QDs的荧光猝灭,然后进行非辐射荧光共振能量转移。与其他测试物种相比,维生素A具有极好的选择性。观察到MPS包覆的ZnS QD的荧光强度与维生素A浓度在3.33-36.66μM范围内,检测限为1.062μM之间存在线性关系。检测机制涉及从ZnS QDs到维生素的光诱导电荷转移,导致ZnS QDs的荧光猝灭,然后进行非辐射荧光共振能量转移。与其他测试物种相比,维生素A具有极好的选择性。观察到MPS包覆的ZnS QD的荧光强度与维生素A浓度在3.33-36.66μM范围内,检测限为1.062μM之间存在线性关系。检测机制涉及从ZnS QDs到维生素的光诱导电荷转移,导致ZnS QDs的荧光猝灭,然后进行非辐射荧光共振能量转移。与其他测试物种相比,维生素A具有极好的选择性。观察到MPS包覆的ZnS QD的荧光强度与维生素A浓度在3.33-36.66μM范围内,检测限为1.062μM之间存在线性关系。
更新日期:2020-01-13
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