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Biofilm Polysaccharide Display Platform: A Natural, Renewable, and Biocompatible Material for Improved Lipase Performance.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-01-22 , DOI: 10.1021/acs.jafc.9b07209
Hao Dong 1 , Wenxue Zhang 1 , Yibing Wang 1 , Dong Liu 2 , Ping Wang 3
Affiliation  

Most of the microorganisms can form biofilms, which makes biofilms an abundant bioresource to be exploited. Due to the limitations of the application of current immobilization methods for biofilms, we developed an immobilization method called the biofilm polysaccharide display (BPD) strategy while maintaining the native biofilm structure and catalytic microenvironment of Clostridium acetobutylicum B3. Lipase Lip181 showed significant improvements in stability after chemical immobilization. For example, immobilized Lip181 retained 74.23% of its original activity after incubation for 14 days, while free Lip181 was totally deactivated. In addition, immobilized Lip181 maintained high residual activity (pH 5.0-11.0), which showed improved resistance to pH changes. Notably, this method did not decrease but slightly increased the relative activity of Lip181 from 6.39 to 6.78 U/mg. Immobilized Lip181 was used to prepare cinnamyl acetate, and it showed a maximum yield of 85.09%. Overall, this biofilm immobilization method may promote the development of biocatalytic and biofilm materials.

中文翻译:

生物膜多糖展示平台:一种天然,可再生且生物相容的材料,可改善脂肪酶的性能。

大多数微生物可以形成生物膜,这使生物膜成为一种可利用的丰富生物资源。由于目前固定化方法在生物膜上的应用存在局限性,我们开发了一种固定化方法,称为生物膜多糖展示(BPD)策略,同时保持了原生的生物膜结构和丙酮丁醇梭菌B3的催化微环境。脂肪酶Lip181在化学固定后显示出明显的稳定性改善。例如,固定化的Lip181在孵育14天后保留其原始活性的74.23%,而游离的Lip181则完全失活。此外,固定化的Lip181保持较高的残留活性(pH 5.0-11.0),显示出对pH变化的抵抗力增强。值得注意的是 该方法没有减少,但将Lip181的相对活性从6.39 U / mg略微增加到6.78 U / mg。固定化的Lip181用于制备乙酸肉桂酯,其最大产率为85.09%。总的来说,这种生物膜固定方法可以促进生物催化和生物膜材料的发展。
更新日期:2020-01-23
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