We propose localizing point-like fluorescent emitters in three dimensions with nanometer precision throughout large volumes using self-interference digital holography (SIDH). SIDH enables imaging of incoherently emitting objects over large axial ranges without refocusing, and single molecule localization techniques allow sub-50 nm resolution in the lateral and axial dimensions. We demonstrate three-dimensional localization with SIDH by imaging 100 and 40 nm fluorescent nanospheres. With 49,000 photons detected, SIDH achieves a localization precision of 5 nm laterally and 40 nm axially. We are able to detect the nanospheres from as few as 13,000 detected photons.

中文翻译：

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利用自干涉数字全息技术对点状物体进行三维纳米尺度定位

我们建议使用自干扰数字全息（SIDH）在三维中以纳米级精度精确定位点状荧光发射器。SIDH可以在不重新聚焦的情况下在较大的轴向范围内对不相干发射的物体成像，单分子定位技术可在横向和轴向尺寸上实现低于50 nm的分辨率。我们通过成像100和40 nm荧光纳米球展示了SIDH的三维定位。SIDH探测到49,000个光子，横向定位精度达到5 nm，轴向定位精度达到40 nm。我们能够从多达13,000个检测到的光子中检测出纳米球。