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Tracking glycosylation in live cells using FTIR spectroscopy.
Talanta ( IF 6.1 ) Pub Date : 2020-01-13 , DOI: 10.1016/j.talanta.2020.120737
Joshua Phelan 1 , Ali Altharawi 2 , K L Andrew Chan 2
Affiliation  

This is the first demonstration of the study of glycan protein turnover in living cells by FTIR with commercially available tetraacetylated N-Azidoacetyl-D-Mannosamine (Ac4ManNAz) label. The FTIR analysis has shown to be able to monitor the metabolism of glycans in living cells in real time. The method is simple, quantitative and requires equipment that are available in many laboratories. It can be used in a wide range of applications such as the study of glycosylation and cell-signalling.

中文翻译:

使用FTIR光谱跟踪活细胞中的糖基化。

这是使用市售的四乙酰化N-叠氮基乙酰基-D-甘露糖胺(Ac4ManNAz)标记通过FTIR研究活细胞中聚糖蛋白更新的第一个证明。FTIR分析显示能够实时监测活细胞中聚糖的代谢。该方法简单,定量并且需要许多实验室中可用的设备。它可以用于广泛的应用中,例如糖基化和细胞信号转导的研究。
更新日期:2020-01-13
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