Polyethylene glycol-conjugated alkylamines - A novel class of surfactants for the saturation of immunoassay solid phase surfaces.,Talanta

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Polyethylene glycol-conjugated alkylamines - A novel class of surfactants for the saturation of immunoassay solid phase surfaces.
Talanta ( IF 5.6 ) Pub Date : 2020-01-13 , DOI: 10.1016/j.talanta.2020.120741
Naho Fujimoto ₁ , Steffen Bade ₁ , Niels Röckendorf ₁ , Katrin Ramaker ₁ , Andreas Frey ₁

Affiliation

All solid-phase immunoassay techniques depend on so-called blocking reagents to suppress the background that is caused by unwanted adhesion of assay system components to the solid support. Commonly used blocking reagents based on biological materials bear severe inherent drawbacks such as heterogeneity and cross-reactivity, while synthetic alternatives often show insufficient background prevention. In this study, polyethylene glycol-conjugated alkylamines were synthesized via a versatile building block approach and were studied as novel blocking reagent candidates in immunoassays. The newly developed substances outperformed commonly used blocking reagents in two different ELISA setups, enabling both, excellent prevention of non-specific binding and particularly high assay sensitivity. This class of surfactants therefore may contribute significantly to the field of assay technology.

中文翻译：

聚乙二醇共轭烷基胺-用于免疫测定固相表面饱和的一类新型表面活性剂。

所有固相免疫测定技术都依赖于所谓的封闭剂来抑制背景，该背景是由于测定系统组分不希望地粘附在固体支持物上而引起的。常用的基于生物材料的封闭剂具有严重的固有缺点，例如异质性和交叉反应性，而合成替代品通常显示出不足的背景预防作用。在这项研究中，聚乙二醇偶联的烷基胺是通过一种通用的构建方法合成的，并在免疫测定中作为新型的封闭剂候选物进行了研究。在两种不同的ELISA设置中，新开发的物质优于常用的封闭剂，从而能够出色地防止非特异性结合，并具有很高的测定灵敏度。

更新日期：2020-01-13

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