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Ratiometric fluorescent probe based on AuNCs induced AIE for quantification and visual sensing of glucose
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2020-04-01 , DOI: 10.1016/j.aca.2020.01.004
Xiaoli Wu 1 , Peiling Wu 1 , Mengying Gu 1 , Jian Xue 1
Affiliation  

Blood glucose measurement plays a very important role in clinical diagnosis and fluorescence analysis has attracted extensive attention. A novel ratiometric fluorescent system with aggregation induced emission (AIE) property for the detection of glucose was established in this work. In this system, bovine serum albumin-stabilized Au nanoclusters (BSA-AuNCs) served both as the fluorescent detection probe and the AIE inducer. An AIE molecule, named sodium 1,2-bis [4-(3-sulfonatopropoxyl) phenyl]-1,2-diphenylethene (BSPOTPE), served as fluorescent reference probe. In the presence of H2O2, the fluorescence intensity of BSPOTPE/BSA-Au NCs at 680 nm progressively decreased while that at 490 nm remained constant. Glucose can be catalyzed by glucose oxidase (GOx) and produces H2O2. Therefore, glucose detection can be conveniently achieved by the proposed strategy. The fluorescence intensity change ratio increased linearly with the glucose concentration in the range 1-8 mM. Moreover, the proposed method exhibits a consecutive fluorescence color change ("from red to cyan") to glucose concentration in the range of 1-8 mM under a 365 nm UV lamp and exhibits bright "red" or bright "cyan" in lower glucose concentrations (lower than 3 mM) or high glucose concentrations (higher than 7 mM), respectively. The work offers an ideal rapid clinical diagnostic method for both normal and abnormal blood glucose screening.

中文翻译:

基于 AuNCs 诱导 AIE 的比例荧光探针用于葡萄糖的定量和视觉传感

血糖测量在临床诊断中起着非常重要的作用,荧光分析引起了广泛的关注。在这项工作中建立了一种具有聚集诱导发射 (AIE) 特性的新型比率荧光系统,用于检测葡萄糖。在该系统中,牛血清白蛋白稳定的 Au 纳米团簇 (BSA-AuNCs) 作为荧光检测探针和 AIE 诱导剂。一种名为 1,2-双 [4-(3-磺基丙氧基) 苯基]-1,2-二苯基乙烯 (BSPOTPE) 钠的 AIE 分子用作荧光参考探针。在 H2O2 存在下,BSPOTPE/BSA-Au NCs 在 680 nm 处的荧光强度逐渐降低,而在 490 nm 处的荧光强度保持不变。葡萄糖可以被葡萄糖氧化酶 (GOx) 催化并产生 H2O2。所以,通过所提出的策略可以方便地实现葡萄糖检测。荧光强度变化率随葡萄糖浓度在 1-8 mM 范围内线性增加。此外,所提出的方法在 365 nm 紫外灯下表现出连续的荧光颜色变化(“从红色到青色”)至葡萄糖浓度在 1-8 mM 范围内,并在较低的葡萄糖中表现出明亮的“红色”或明亮的“青色”浓度(低于 3 mM)或高葡萄糖浓度(高于 7 mM)。这项工作为正常和异常血糖筛查提供了一种理想的快速临床诊断方法。所提出的方法在 365 nm 紫外线灯下表现出连续的荧光颜色变化(“从红色到青色”)至葡萄糖浓度范围为 1-8 mM,并在较低的葡萄糖浓度下表现出明亮的“红色”或明亮的“青色”(低于 3 mM)或高葡萄糖浓度(高于 7 mM)。这项工作为正常和异常血糖筛查提供了一种理想的快速临床诊断方法。所提出的方法在 365 nm 紫外线灯下表现出连续的荧光颜色变化(“从红色到青色”)至葡萄糖浓度范围为 1-8 mM,并在较低的葡萄糖浓度下表现出明亮的“红色”或明亮的“青色”(低于 3 mM)或高葡萄糖浓度(高于 7 mM)。这项工作为正常和异常血糖筛查提供了一种理想的快速临床诊断方法。
更新日期:2020-04-01
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