Visualization of the structure of the Endoplasmic Reticulum (ER) in living cells is important for the understanding of its function. Here we synthesized a library of BODIPY labeled 8 hydroxyquinoline derivatives and evaluated their spectroscopic properties, cytotoxicity, and intracellular localization. The compounds were easily obtained in 34–80% yield. Based on the spectral properties and low cytotoxicity, we selected the quinolin-8-yl pentanoate derivative 17 for in vivo labeling of the ER. Fluorescence staining of the ER was evaluated by comparison with a commercial ER tracker. The molecules here developed are smaller than the alternatives commercially available while still presenting a high specificity towards the ER.

可视化活细胞内质网（ER）的结构对于理解其功能很重要。在这里，我们合成了BODIPY标记的8个羟基喹啉衍生物的文库，并评估了它们的光谱性质，细胞毒性和细胞内定位。这些化合物很容易以34–80％的产率获得。基于光谱特性和低细胞毒性，我们选择了喹啉-8-戊酸酯衍生物17用于ER的体内标记。ER的荧光染色是通过与商用ER示踪剂进行比较来评估的。这里开发的分子比市售的替代品小，同时仍对ER表现出高特异性。