In order to discover new antibiotics with improved activity and selectivity, we created a reliable in vitro reporter system to detect trans-translation activity, the main mechanism for recycling ribosomes stalled on problematic messenger RNA (mRNA) in bacteria. This system is based on an engineered tmRNA variant that reassembles the green fluorescent protein (GFP) when trans-translation is active. Our system is adapted for high-throughput screening of chemical compounds by fluorescence.

中文翻译：

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重新组装绿色荧光蛋白，用于体外翻译评估。

为了发现具有改善的活性和选择性的新抗生素，我们创建了一个可靠的体外报告系统来检测反翻译活性，这是回收滞留在细菌中有问题的信使RNA（mRNA）上的核糖体的主要机制。该系统基于工程化的tmRNA变体，当转译激活时，该变体会重新组装绿色荧光蛋白（GFP）。我们的系统适用于通过荧光高通量筛选化合物。