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Effects of Growth Conditions and Cultivar on the Content and Physiochemical Properties of Arabinoxylan in Barley.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-01-17 , DOI: 10.1021/acs.jafc.9b05488
Ombretta Marconi 1, 2 , Ivan Tomasi 1 , Valeria Sileoni 2 , Umberto Bonciarelli 1 , Marcello Guiducci 1 , Stefano Maranghi 2 , Giuseppe Perretti 1, 2
Affiliation  

The present study aims to evaluate the effect of the growth conditions and the cultivar on the total and water-extractable (W-E) arabinoxylan (AX) in barley. For this purpose, nine barley varieties from two different years were analyzed. The total AX content ranged from 5.97 to 8.98 wt % d.m., while the W-E AX ranged from 0.06 to 0.35 wt % d.m. The W-E AX molecular properties were characterized by high-pressure size exclusion chromatography (HPSEC)-triple detector array (TDA). The molecular weight was between 2.3 × 105 and 12.6 × 105 Da, the polydispersity was between moderate and broad (1.1 < Mw/Mn < 4.3), and the conformation was a stiff semiflexible coil (0.5 < α < 1.3). The results indicate that the year influences the content of total AX and W-E AX and some molecular characteristics of W-E AX, such as its polydispersity and its conformation. Finally, the results demonstrated that the W-E AX can be used as an index of the malting attitude of barley because it positively correlates with germinative energy and kernel dimension.

中文翻译:

生长条件和品种对大麦中阿拉伯木聚糖含量和理化特性的影响。

本研究旨在评估生长条件和品种对大麦中总的和水可提取的(WE)阿拉伯木聚糖(AX)的影响。为此,分析了两个不同年份的九种大麦品种。总AX含量范围为5.97至8.98 wt%dm,而WE AX范围为0.06至0.35 wt%dm。WE AX分子性质通过高压尺寸排阻色谱法(HPSEC)-三重检测器阵列(TDA)表征。分子量在2.3×105和12.6×105 Da之间,多分散度在中等和宽范围内(1.1 <Mw / Mn <4.3),构象是刚性的半柔性线圈(0.5 <α<1.3)。结果表明,年份影响总AX和WE AX的含量以及WE AX的一些分子特性,例如其多分散性及其构象。最后,结果表明,WE AX可以用作大麦发芽状态的指标,因为它与发芽能量和籽粒尺寸呈正相关。
更新日期:2020-01-21
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