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Metabolic Profiling and Transcriptome Analysis of Mulberry Leaves Provide Insights into Flavonoid Biosynthesis.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-01-22 , DOI: 10.1021/acs.jafc.9b06931 Dong Li 1 , Guo Chen 1 , Bi Ma 1 , Chengzhang Zhong 1 , Ningjia He 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-01-22 , DOI: 10.1021/acs.jafc.9b06931 Dong Li 1 , Guo Chen 1 , Bi Ma 1 , Chengzhang Zhong 1 , Ningjia He 1
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Flavonoids are widely distributed in mulberry leaves and have been recognized for their beneficial physiological effects on the human health. Here, we analyzed variations in 44 flavonoid compounds among 91 mulberry resources. Metabolic profiling revealed that O-rhamnosylated flavonols and malonylated flavonol glycosides, including rutin and quercetin 3-O-(6″-O-malonylglucoside) (Q3MG), were absent from Morus notabilis and multiple mulberry (Morus alba L.) resources. Transcriptome and phylogenetic analyses of flavonoid-related UDP-glycosyltransferases (UGTs) suggested that the flavonol 3-O-glucoside-O-rhamnosyltransferase (FGRT) KT324624 is a key enzyme involved in rutin synthesis. A recombinant FGRT protein was able to convert kaempferol/quercetin 3-O-glucoside to kaempferol 3-O-rutinoside (K3G6″Rha) and rutin. The recombinant FGRT was able to use 3-O-glucosylated flavonols but not flavonoid aglycones or 7-O-glycosylated flavonoids as substrates. The enzyme preferentially used UDP-rhamnose as the sugar donor, indicating that it was a flavonol 3-O-glucoside: 6″-O-rhamnosyltransferase. This study provided insights into the biosynthesis of rutin in mulberry.
中文翻译：
桑叶的代谢分析和转录组分析提供了对类黄酮生物合成的见解。
黄酮类化合物广泛分布于桑叶中，并因其对人体健康的有益生理作用而得到公认。在这里，我们分析了91种桑树资源中44种黄酮类化合物的变异。代谢谱分析表明，桑us和多种桑resources资源中都不含O-鼠李糖基黄酮醇和丙二酰黄酮醇苷，包括芦丁和槲皮素3-O-（6″ -O-丙二酰葡萄糖苷）（Q3MG）。类黄酮相关的UDP-糖基转移酶（UGT）的转录组和系统发育分析表明，黄酮醇3-O-糖苷-O-鼠李糖基转移酶（FGRT）KT324624是参与芦丁合成的关键酶。重组FGRT蛋白能够将山ka酚/槲皮素3-O-葡糖苷转化为山ka酚3-O-芸香苷（K3G6″ Rha）和芦丁。重组FGRT能够使用3-O-葡萄糖基化的黄酮醇而不是黄酮类苷元或7-O-糖基化的类黄酮作为底物。该酶优先使用UDP-鼠李糖作为糖供体，表明它是黄酮醇3-O-葡糖苷：6″ -O-鼠李糖基转移酶。该研究为桑树中芦丁的生物合成提供了见识。
更新日期：2020-01-23
中文翻译：
桑叶的代谢分析和转录组分析提供了对类黄酮生物合成的见解。
黄酮类化合物广泛分布于桑叶中，并因其对人体健康的有益生理作用而得到公认。在这里，我们分析了91种桑树资源中44种黄酮类化合物的变异。代谢谱分析表明，桑us和多种桑resources资源中都不含O-鼠李糖基黄酮醇和丙二酰黄酮醇苷，包括芦丁和槲皮素3-O-（6″ -O-丙二酰葡萄糖苷）（Q3MG）。类黄酮相关的UDP-糖基转移酶（UGT）的转录组和系统发育分析表明，黄酮醇3-O-糖苷-O-鼠李糖基转移酶（FGRT）KT324624是参与芦丁合成的关键酶。重组FGRT蛋白能够将山ka酚/槲皮素3-O-葡糖苷转化为山ka酚3-O-芸香苷（K3G6″ Rha）和芦丁。重组FGRT能够使用3-O-葡萄糖基化的黄酮醇而不是黄酮类苷元或7-O-糖基化的类黄酮作为底物。该酶优先使用UDP-鼠李糖作为糖供体，表明它是黄酮醇3-O-葡糖苷：6″ -O-鼠李糖基转移酶。该研究为桑树中芦丁的生物合成提供了见识。
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