We propose a novel absolute calibrate method for digital holographic microscopy with the sequential shift method using Chebyshev polynomials. We separate the object phase and the aberrations by sequential shifting the sample twice in vertical plane of the optical axis. The aberrations phase is then calculated using the high order Chebyshev polynomials. The correct phase is obtained by subtracting the aberrations from the original phase containing the aberration. This method can compensate for the complex aberrations including high-order aberrations without changing the traditional optical system. Meanwhile, it can effectively protect the medium and high frequency information of the specimen in the phase image. Numerical simulation and experimental results demonstrate the availability and advantages of the absolute calibrate method.

中文翻译：

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基于切比雪夫多项式拟合的数字全息相位成像中的连续位移绝对相位像差校准

我们提出了一种新的数字全息显微镜绝对校准方法，使用切比雪夫多项式的顺序移位方法。我们通过在光轴的垂直平面上连续移动样品两次来分离物相和像差。然后使用高阶切比雪夫多项式计算像差相位。正确的相位是通过从包含像差的原始相位中减去像差来获得的。这种方法可以在不改变传统光学系统的情况下，对包括高阶像差在内的复杂像差进行补偿。同时，它可以有效地保护相位图像中标本的中高频信息。数值模拟和实验结果证明了绝对校准方法的可用性和优势。