Parallel evolutionary paths to produce more than one Pseudomonas aeruginosa biofilm phenotype.,npj Biofilms and Microbiomes

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Parallel evolutionary paths to produce more than one Pseudomonas aeruginosa biofilm phenotype.
npj Biofilms and Microbiomes ( IF 7.8 ) Pub Date : 2020-01-10 , DOI: 10.1038/s41522-019-0113-6
Janne G Thöming _{1,

2} , Jürgen Tomasch ₃ , Matthias Preusse ₃ , Michal Koska ₁ , Nora Grahl _{1,

3} , Sarah Pohl _{1,

3} , Sven D Willger _{1,

3} , Volkhard Kaever ₄ , Mathias Müsken _{1,

3,

5} , Susanne Häussler _{1,

2,

3,

6}

Affiliation

Studying parallel evolution of similar traits in independent within-species lineages provides an opportunity to address evolutionary predictability of molecular changes underlying adaptation. In this study, we monitored biofilm forming capabilities, motility, and virulence phenotypes of a plethora of phylogenetically diverse clinical isolates of the opportunistic pathogen Pseudomonas aeruginosa. We also recorded biofilm-specific and planktonic transcriptional responses. We found that P. aeruginosa isolates could be stratified based on the production of distinct organismal traits. Three major biofilm phenotypes, which shared motility and virulence phenotypes, were produced repeatedly in several isolates, indicating that the phenotypes evolved via parallel or convergent evolution. Of note, while we found a restricted general response to the biofilm environment, the individual groups of biofilm phenotypes reproduced biofilm transcriptional profiles that included the expression of well-known biofilm features, such as surface adhesive structures and extracellular matrix components. Our results provide insights into distinct ways to make a biofilm and indicate that genetic adaptations can modulate multiple pathways for biofilm development that are followed by several independent clinical isolates. Uncovering core regulatory pathways that drive biofilm-associated growth and tolerance towards environmental stressors promises to give clues to host and environmental interactions and could provide useful targets for new clinical interventions.

中文翻译：

产生不止一种铜绿假单胞菌生物膜表型的平行进化路径。

研究独立的种内谱系中相似性状的平行进化，为解决适应背后的分子变化的进化可预测性提供了机会。在这项研究中，我们监测了大量系统发育多样化的机会性病原体铜绿假单胞菌临床分离株的生物膜形成能力、运动性和毒力表型。我们还记录了生物膜特异性和浮游转录反应。我们发现铜绿假单胞菌分离株可以根据不同生物性状的产生进行分层。三种主要的生物膜表型，具有共同的运动性和毒力表型，在几个分离株中重复产生，表明这些表型是通过平行或趋同进化而进化的。值得注意的是，虽然我们发现对生物膜环境的一般反应有限，但生物膜表型的各个组再现了生物膜转录谱，其中包括众所周知的生物膜特征的表达，例如表面粘附结构和细胞外基质成分。我们的研究结果提供了对形成生物膜的不同方式的见解，并表明遗传适应可以调节生物膜发育的多种途径，随后产生几种独立的临床分离株。揭示驱动生物膜相关生长和对环境压力源耐受的核心调控途径有望为宿主和环境相互作用提供线索，并为新的临床干预措施提供有用的目标。

更新日期：2020-01-10

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