Antibiotics are the most consumed therapeutic classes worldwide and are released to the environment in their original form as well as potentially active metabolites and/or degradation products. Consequences of the occurrence of these compounds in the environment are primarily related to bacterial resistance development.

This work presents a validated analytical method based on solid phase extraction (SPE) using HLB cartridges, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) for quantification of seven different fluoroquinolone antibiotics, namely ciprofloxacin (CPF), enrofloxacin (ENR), lomefloxacin (LOM), norfloxacin (NOR), ofloxacin (OFL), prulifloxacin (PLF) and moxifloxacin (MOX) and its application to detect the target compounds in influents and effluents of wastewater treatment plants (WWTP). Linearity was established through calibration curves in solvent and matrix match using internal calibration method in the range of 50–1300 ng L⁻¹ and all the fluoroquinolones showed good linear fit (r² ≥ 0.991). Accuracy ranged between 80.3 and 92.9% and precision was comprised between 7.2 and 14.6%, and 10.7 and 18.1% in intra- and inter-batch determinations, respectively. Method detection and quantification limits ranged from 6.7 to 59.0 ng L⁻¹ and 22.3–196.6 ng L⁻¹, respectively.

Influents and effluents of fifteen WWTPs of North of Portugal were analyzed. OFL was the fluoroquinolone found at the highest concentration, up to 4587.0 ng L⁻¹ and 987.9 ng L⁻¹, in influent and effluent, respectively. NOR and PLF were not detected.

抗生素是世界上最消耗的治疗类，并且以其原始形式以及潜在的活性代谢产物和/或降解产物释放到环境中。这些化合物在环境中出现的后果主要与细菌耐药性的发展有关。

这项工作提出了一种有效的分析方法，该方法基于一种使用HLB柱的固相萃取（SPE），然后进行液相色谱-串联质谱（LC-MS / MS）定量的七种不同的氟喹诺酮抗生素，即环丙沙星（CPF），恩诺沙星ENR），洛美沙星（LOM），诺氟沙星（NOR），氧氟沙星（OFL），普鲁沙星（PLF）和莫西沙星（MOX）及其在检测废水处理厂（WWTP）进水和出水中的目标化合物中的应用。通过使用内部校准方法在50–1300 ng L ^-1范围内的溶剂和基质匹配的校准曲线建立线性，所有氟喹诺酮类药物均显示出良好的线性拟合（r ² ≥0.991）。批内和批间测定的准确度分别在80.3％和92.9％之间，准确度在7.2％和14.6％之间，以及10.7％和18.1％之间。方法的检测限和定量限分别为6.7至59.0 ng L ^-1和22.3-196.6 ng L ^-1。

分析了葡萄牙北部15个污水处理厂的进水和出水。OFL是氟喹诺酮发现在最高浓度，高达4587.0纳克大号^-1和987.9纳克大号^-1分别在流入和流出。未检测到NOR和PLF。