Electrochemical immunosensor based on carboxylated single‐walled carbon nanotube‐chitosan functional layer for the detection of cephalexin,Food Science & Nutrition

当前位置： X-MOL 学术 › Food Sci. Nutr. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Electrochemical immunosensor based on carboxylated single‐walled carbon nanotube‐chitosan functional layer for the detection of cephalexin
Food Science & Nutrition ( IF 3.5 ) Pub Date : 2020-01-09 , DOI: 10.1002/fsn3.1382
Wenlong Yu ₁ , Yaxin Sang ₁ , Tianying Wang ₁ , Weihua Liu ₁ , Xianghong Wang ₁

Affiliation

In this study, a sensitive and selective electrochemical immunosensor for cephalexin (CEX) determination on a glassy carbon electrode (GCE) surface was modified by a carboxylated single‐walled carbon nanotubes/chitosan (SWNTs‐COOH/CS) composite. The SWNTs‐COOH/CS composite was used to enhance sensor performance and to enlarge the electrochemical response of CEX. The cephalosporin‐ovalbumin coupling (CEX‐OVA) was synthesized using the reactive ester method. The free CEX in solution could be effectively measured based on the competitive immunoreaction between CEX‐antibody and CEX. Under optimal conditions, the electrochemical immunosensor offered an excellent response for CEX. The linear range was 1–800 ng/ml, with a detection limit of 45.7 ng/ml (S/N = 3). This method was applied to determine CEX in six different samples and obtained the recovery range from 80.15% to 94.04%. These results indicated that the fabricated electrochemical immunosensor and sensing method are suitable for quantification of CEX in real samples. These have great potential for wider applications in environmental and agri‐food products industries.

中文翻译：

基于羧化单壁碳纳米管-壳聚糖功能层的电化学免疫传感器检测头孢氨苄

在这项研究中，通过羧基化单壁碳纳米管/壳聚糖（SWNTs-COOH/CS）复合材料修饰了一种灵敏、选择性的电化学免疫传感器，用于在玻碳电极（GCE）表面上测定头孢氨苄（CEX）。 SWNTs-COOH/CS 复合材料用于增强传感器性能并扩大 CEX 的电化学响应。采用反应酯法合成了头孢菌素-卵白蛋白偶联物（CEX-OVA）。基于CEX抗体与CEX之间的竞争性免疫反应，可以有效测量溶液中的游离CEX。在最佳条件下，电化学免疫传感器为 CEX 提供了出色的响应。线性范围为 1–800 ng/ml，检测限为 45.7 ng/ml ( S/N = 3)。应用该方法测定了6个不同样品中的CEX，回收率范围为80.15%~94.04%。这些结果表明所制造的电化学免疫传感器和传感方法适用于实际样品中 CEX 的定量。这些在环境和农产品行业具有更广泛应用的巨大潜力。

更新日期：2020-01-09

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11