A multiclass and multiresidue method for screening veterinary drugs and pesticides in infant formula was developed and validated using ultrahigh-performance liquid chromatography coupled to Quadrupole-Orbitrap high-resolution mass spectrometry (UHPLC-HRMS). A total of 49 veterinary drugs and pesticides investigated belong to 11 classes including antivirals, anticoccidials, macrolides, pyrethroids, insecticides, sulfonamides, beta-agonists, sedatives, thyreostats, nonsteroidal anti-inflammatory drugs, and other pharmacologically active substances. A generic sample preparation and highly selective acquisition mode of parallel reaction monitoring (PRM) were deliberately incorporated to perform efficient screening analysis. As a result, the screening target concentrations of the analytes varied from 1 to 500 μg/kg with ≤5% of false compliant rate as specified in Decision 2002/657/EC for screening analysis. The average recoveries ranged from 40.7 to 124.9% as well as the relative standard deviations from 4.2 to 26.6%, respectively. The matrix effects and interferences were effectively controlled by integrated application of dispersive solid phase extraction, PRM scan mode, and matrix-matched standard calibration. The proposed method will be helpful to provide applicable strategy for screening residues in infant formula with surveillance purpose.

中文翻译：

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使用具有PRM扫描模式的Quadrupole-Orbitrap MS对婴儿配方食品中的兽药和农药进行多类和多残留筛选。

建立了一种多级，多残留的婴幼儿配方奶粉中兽药和农药筛查方法，并通过超高效液相色谱与四极杆Orbitrap高分辨率质谱联用（UHPLC-HRMS）进行了验证。总共调查的49种兽药和农药属于11类，包括抗病毒药，抗球虫药，大环内酯类，拟除虫菊酯，杀虫剂，磺酰胺，β-激动剂，镇静剂，甲状腺调节剂，非甾体类抗炎药和其他药理活性物质。故意并入通用样品制备和高度选择性的平行反应监控（PRM）采集模式以进行有效的筛选分析。结果是，分析物的筛查目标浓度为1至500μg/ kg，决策2002/657 / EC中规定的筛查分析错误符合率≤5％。平均回收率分别为40.7至124.9％，相对标准偏差为4.2至26.6％。通过集成应用分散固相萃取，PRM扫描模式和基质匹配的标准校准，可以有效地控制基质效应和干扰。所提出的方法将有助于为监测婴儿配方奶粉中的残留物提供适用的策略。通过集成应用分散固相萃取，PRM扫描模式和基质匹配的标准校准，可以有效地控制基质效应和干扰。所提出的方法将有助于为监测婴儿配方奶粉中的残留物提供适用的策略。通过集成应用分散固相萃取，PRM扫描模式和基质匹配的标准校准，可以有效地控制基质效应和干扰。所提出的方法将有助于为监测婴儿配方奶粉中的残留物提供适用的策略。