Abnormal Liquid Chasing Effect in Paper Capillary Enables Versatile Gradient Generation on Microfluidic Paper Analytical Devices.,Analytical Chemistry

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Abnormal Liquid Chasing Effect in Paper Capillary Enables Versatile Gradient Generation on Microfluidic Paper Analytical Devices.
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-01-17 , DOI: 10.1021/acs.analchem.9b04934
Yu Liu ₁ , Jin-Wen Shangguan ₁ , Bi-Yi Xu _{1,

2} , Xiao-Dong Yu ₁ , Jing-Juan Xu ₁ , Hong-Yuan Chen ₁

Affiliation

Microfluidic paper analytical devices (μPADs) are smart and accessible substituents to traditional counterparts in point-of-care tests (POCT), which exploited delicate control over passive fluid in microscale for rich functions. In this work, we are extending such control by introducing novel ways to generate 1D and 2D gradients on μPADs. It is achieved by using paper-capillary-based serial sampling. The paper capillary is composed of a concaved paper channel sealed with tape, with test pads properly distributed aside. In such a structure, the liquid can not only quickly and automatically flow along the capillary but also be continuously consumed by the peripheral test pads. Thus, when we do serial sampling, an abnormal liquid chasing effect can be observed, where the later liquid sample chases and surpasses the earlier parts in the paper capillary, leading to reversely ordered sample distribution compared with that in a typical glass capillary. This specialty allows for fast, ordered, and tunable sequential sampling and enables efficient generation of 1D and 2D concentration gradient with one, two, and even three components on μPADs. Besides, we verified the applicability of this technique for arrayed assays, including 1D serial dilution-based metal ion colorimetry and a 2D bacterial antibiotic susceptibility test for synergic effect evaluations, which paves the way for high-throughput sample analysis and information-rich condition screening on μPADs.

中文翻译：

纸毛细管中的异常液体追逐效应可在微流纸分析设备上实现多种梯度生成。

微流纸分析设备（μPAD）是即时检验（POCT）中传统同类产品的智能且易于使用的替代品，该产品利用微级被动流体的精细控制来实现丰富功能。在这项工作中，我们通过引入在μPAD上生成1D和2D梯度的新颖方法来扩展这种控制。这是通过使用基于纸质毛细管的串行采样实现的。纸毛细管由用胶带密封的凹入纸通道组成，测试垫正确放置在一边。在这样的结构中，液体不仅可以快速且自动地沿着毛细管流动，而且可以被外围测试垫连续地消耗。因此，当我们进行连续采样时，可以观察到异常的液体追赶效果，较晚的液体样品追赶并超过纸毛细管中较早的部分，与典型的玻璃毛细管相比，导致样品分配的顺序相反。该专业技术可实现快速，有序和可调的顺序采样，并能有效生成μPAD上具有一个，两个甚至三个分量的一维和二维浓度梯度。此外，我们验证了该技术在阵列分析中的适用性，包括基于一维系列稀释的金属离子比色法和用于协同效应评估的二维细菌抗生素敏感性测试，为高通量样品分析和信息丰富的条件筛选铺平了道路。在μPAD上。以及可调整的顺序采样，并能够在μPAD上高效生成一维和二维浓度梯度，其中包含一个，两个甚至三个成分。此外，我们验证了该技术在阵列分析中的适用性，包括基于一维系列稀释的金属离子比色法和用于协同效应评估的二维细菌抗生素敏感性测试，为高通量样品分析和信息丰富的条件筛选铺平了道路。在μPAD上。以及可调整的顺序采样，并能够在μPAD上高效生成一维和二维浓度梯度，其中包含一个，两个甚至三个成分。此外，我们验证了该技术在阵列分析中的适用性，包括基于一维系列稀释的金属离子比色法和用于协同效应评估的二维细菌抗生素敏感性测试，为高通量样品分析和信息丰富的条件筛选铺平了道路。在μPAD上。

更新日期：2020-01-17

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