Extrusion of RNA from a DNA-Origami-Based Nanofactory.,ACS Nano

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Extrusion of RNA from a DNA-Origami-Based Nanofactory.
ACS Nano ( IF 15.8 ) Pub Date : 2020-01-17 , DOI: 10.1021/acsnano.9b06466
Jaeseung Hahn _{1,

2,

3} , Leo Y T Chou _{2,

3} , Rasmus S Sørensen _{2,

3} , Richard M Guerra _{2,

3} , William M Shih _{2,

3}

Affiliation

Cells often spatially organize biomolecules to regulate biological interactions. Synthetic mimicry of complex spatial organization may provide a route to similar levels of control for artificial systems. As a proof-of-principle, we constructed an RNA-extruding nanofactory using a DNA-origami barrel with an outer diameter of 60 nm as a chassis for integrated rolling-circle transcription and processing of RNA through spatial organization of DNA templates, RNA polymerases, and RNA endonucleases. The incorporation efficiency of molecular components was quantified to be roughly 50% on designed sites within the DNA-origami chassis. Each integrated nanofactory with RNA-producing units, composed of DNA templates and RNA polymerases, produced 100 copies of target RNA in 30 min on average. Further integration of RNA endonucleases that cleave rolling-circle transcripts from concatemers into monomers resulted in 30% processing efficiency. Disabling spatial organization of molecular components on DNA origami resulted in suppression of RNA production as well as processing.

中文翻译：

从基于DNA折纸的纳米工厂中提取RNA。

细胞通常在空间上组织生物分子以调节生物相互作用。复杂的空间组织的合成模仿可能为人造系统的相似控制水平提供一条途径。作为原理的证明，我们使用外径为60 nm的DNA折纸桶作为底盘，通过DNA模板的空间组织，RNA聚合酶的空间组织整合RNA的循环转录和加工，构建了RNA挤出纳米工厂。和RNA核酸内切酶。在DNA折纸底盘的设计位点上，分子成分的结合效率被量化为大约50％。每个具有RNA产生单元的集成纳米工厂（由DNA模板和RNA聚合酶组成）平均在30分钟内产生100份靶RNA。RNA核酸内切酶的进一步整合将滚环转录物从串联体切割成单体，从而使加工效率提高了30％。禁用DNA折纸上分子成分的空间组织会导致RNA产生和加工受到抑制。

更新日期：2020-01-21

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