Lanthanide-Binding Tags for 3D X-Ray Imaging of Proteins in Cells at Nanoscale Resolution,Journal of the American Chemical Society

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Lanthanide-Binding Tags for 3D X-Ray Imaging of Proteins in Cells at Nanoscale Resolution
Journal of the American Chemical Society ( IF 14.4 ) Pub Date : 2020-01-10 , DOI: 10.1021/jacs.9b11571
Tiffany W Victor _{1,

2} , Katherine H O'Toole ₃ , Lindsey M Easthon ₃ , Mingyuan Ge ₁ , Randy J Smith ₁ , Xiaojing Huang ₁ , Hanfei Yan ₁ , Yong S Chu ₁ , Si Chen ₄ , Doga Gursoy _{4,

5} , Martina Ralle ₆ , Barbara Imperiali ₇ , Karen N Allen ₃ , Lisa M Miller _{1,

2}

Affiliation

We report the application of lanthanide-binding tags (LBTs) for two- and three-dimensional X-ray imaging of individual proteins in cells with a sub-15 nm beam. The method combines encoded LBTs, which are tags of minimal size (ca 15-20 amino acids) affording high-affinity lanthanide ion binding, and X-ray Fluorescence Microscopy (XFM). This approach enables visualization of LBT-tagged proteins while simultaneously measuring the elemental distribution in cells at a spatial resolution necessary for visualizing cell membranes and eukaryotic subcellular organelles.

中文翻译：

用于以纳米级分辨率对细胞中的蛋白质进行 3D X 射线成像的镧系元素结合标签

我们报告了镧系元素结合标签 (LBT) 在具有亚 15 nm 光束的细胞中单个蛋白质的二维和三维 X 射线成像中的应用。该方法结合了编码的 LBT，它们是最小尺寸（约 15-20 个氨基酸）的标签，可提供高亲和力的镧系元素离子结合，以及 X 射线荧光显微镜 (XFM)。这种方法可以实现 LBT 标记蛋白的可视化，同时以可视化细胞膜和真核亚细胞器所需的空间分辨率测量细胞中的元素分布。

更新日期：2020-01-10

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