There is disputable on the role of nitrilase-like 2 (NIT2) in cancer. Its expression and its relationship with clinicopathological features in tongue squamous cell carcinoma (TSCC) are not yet clear. The purpose of this study is to investigate the expression of NIT2 in TSCC and its correlation with clinicopathological characteristics in TSCC patients. Through proteomic identification, we found that the protein NIT2 was related to the development of TSCC. q-PCR, western blot and immunohistochemistry techniques were applied to detect the expression of NIT2 in TSCC. The relationship between the expression of NIT2 and clinicopathological features was analyzed by Chi square tests. The results showed the expression of NIT2 in TSCC was significantly higher than that in normal tongue tissues (p < 0.05). Univariate and multivariate analysis showed that the positive expression of NIT2 and N classification were associated with decreased disease-free survival rate (DFS) and overall survival (OS) (p < 0.05). The results suggested that NIT2 is overexpressed in TSCC and NIT2 may be a potential therapeutic target for TSCC.

中文翻译：

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NIT2过度表达预示舌鳞状细胞癌患者预后不良。

关于腈水解酶样2（NIT2）在癌症中的作用存在争议。在舌鳞状细胞癌（TSCC）中它的表达及其与临床病理特征的关系尚不清楚。这项研究的目的是调查TSCC患者中NIT2的表达及其与临床病理特征的相关性。通过蛋白质组学鉴定，我们发现蛋白质NIT2与TSCC的发展有关。采用q-PCR，western blot和免疫组化技术检测NIT2在TSCC中的表达。通过卡方检验分析了NIT2的表达与临床病理特征之间的关系。结果表明，TSCC中NIT2的表达明显高于正常舌组织（p <0.05）。单因素和多因素分析表明，NIT2和N分类的阳性表达与无病生存率（DFS）和总体生存率（OS）降低相关（p <0.05）。结果表明，NIT2在TSCC中过表达，NIT2可能是TSCC的潜在治疗靶标。